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Invitrogen
Rat IgG2a kappa Isotype Control (eBR2a), Pacific Orange™, eBioscience™
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FIGURE: 1 / 1
Rat IgG2a kappa Isotype Control (79-4321-81) in Flow
C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, eFluor 450 (Product # 48-0031-82) and staining buffer (autofluorescence) (left) or 0.125 µg of Rat IgG2a kappa Isotype Control, Pacific Orange (right). Cells in the lymphocyte gate were used for analysis.
Product Details
79-4321-81
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBR2a
Conjugate
Excitation/Emission Max
400/551 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815355
Product Specific Information
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis.
Applications Tested: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells and should be used at the same concentration as the experimental antibody.
Pacific Orange emits at 551 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 405 nm; Emission: 551 nm; Laser: Violet Laser.
Target Information
Rat IgG2a is an immunoglobulin subclass that is commonly used in research and diagnostic applications. It is produced by the rat immune system and is known for its ability to activate complement and mediate antibody-dependent cell-mediated cytotoxicity (ADCC). Rat IgG2a has a molecular weight of approximately 160 kDa and is composed of two heavy chains and two light chains. It is often used as a control or reference antibody in experiments involving rat models, and is also commonly used in flow cytometry and immunohistochemistry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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