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Invitrogen
Rat anti-Mouse IgG2b Secondary Antibody, FITC
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Product Details
SA1-35649
Applications
Tested Dilution
Immunohistochemistry (Frozen) (IHC (F))
Assay-dependent
ELISA (ELISA)
Assay-dependent
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG, kappa
Class
Monoclonal
Type
Secondary Antibody
Clone
LO-MG2b-2
Immunogen
Immunoglobulins.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1076013
Target
IgG2b
Antibody Form
Whole Antibody
Product Specific Information
Mouse Gamma 2b Heavy Chain of Immunoglobulin (determined by immunodot). positively tested on BALB/c and C57BL/6 mouse sera. The specificity of every rat monoclonal antibody anti-mouse IgG subclasses is determined in a range of optimal concentrations. Increasing the first or the second antibody at an unnecessary too high concentration can induce possible cross-reactions with other mouse IgG subclasses. Avidity on IgG2b: 1 * 1010 M-1. No cross-reactivity with chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG and human IgG or IgM (ELISA test). Available unconjugated MON5055, FITC MON5055F, Biotin MON5055B, PE MON5055P
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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