Invitrogen

Rb Recombinant Rabbit Monoclonal Antibody (2R4M3)

View all (119) Rb antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-51348

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2R4M3

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 750-850 of human RB1 (NP_000312.2).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3093814

Product Specific Information

Immunogen sequence: DSIIVFYNSV FMQRLKTNIL QYASTRPPTL SPIPHIPRSP YKFPSSPLRI PGGNIYISPL KSPYKISEGL PTPTKMTPRS RILVSIGESF GTSEKFQKIN Q

Target Information

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cleaved Rb; delta RB-p70; DRb-p70; P105RB; p110-RB1; Phospho-Retinoblastoma; pp105; pRb; Rb; Rb-p70; Retin; Retinoblastoma; Retinoblastoma 1 (including osteosarcoma); retinoblastoma gene; Retinoblastoma-associated protein

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Gene Aliases: pRb; Rb; Rb-1; Rb1

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UniProt ID: (Rat) P33568, (Mouse) P13405

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Entrez Gene ID: (Rat) 24708, (Mouse) 19645

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Function(s)
core promoter binding RNA polymerase II activating transcription factor binding DNA binding protein binding transcription factor binding enzyme binding kinase binding ubiquitin protein ligase binding identical protein binding phosphoprotein binding
Process(es)
G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of protein kinase activity cell cycle arrest Ras protein signal transduction spermatogenesis regulation of mitotic cell cycle negative regulation of cell proliferation chromatin modification neuron differentiation sister chromatid biorientation neuron projection development maintenance of mitotic sister chromatid cohesion glial cell apoptotic process skeletal muscle cell differentiation neuron maturation negative regulation of apoptotic process enucleate erythrocyte differentiation regulation of lipid kinase activity myoblast differentiation positive regulation of macrophage differentiation negative regulation of cell cycle positive regulation of mitotic metaphase/anaphase transition negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter digestive tract development cell morphogenesis involved in neuron differentiation negative regulation of epithelial cell proliferation striated muscle cell differentiation cell division neuron apoptotic process regulation of cell cycle protein localization to chromosome, centromeric region cellular response to xenobiotic stimulus regulation of cohesin loading negative regulation of transcription involved in G1/S transition of mitotic cell cycle hepatocyte apoptotic process negative regulation of G1/S transition of mitotic cell cycle regulation of transcription, DNA-templated cell cycle negative regulation of hepatocyte apoptotic process
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