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Invitrogen
RbAp46/RbAp48 Monoclonal Antibody (15G12)
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RbAp46/RbAp48 Antibody (MA1-23277) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of RbAp46/RbAp48 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.
Product Details
MA1-23277
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
15G12
Immunogen
The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2177637
Product Specific Information
Recommended positive controls: K562, THP-1, HL-60.
Predicted molecular weight is 47.6kDa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CAF-1 p48 subunit; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; G1/S transition control protein-binding protein; G1/S transition control protein-binding protein RbAp46; Histone acetyltransferase type B subunit 2; Histone-binding protein RBBP4; Histone-binding protein RBBP7; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP46; Nucleosome-remodeling factor subunit RBAP48; RBBP-4; RBBP-7; retinoblastoma binding protein 4; retinoblastoma binding protein 7; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein 7; Retinoblastoma-binding protein p46; Retinoblastoma-binding protein p48; retinoblastoma-binding protein RbAp46
Gene Aliases: AA409861; AI173248; AU019541; BB114024; lin-53; mRbAp46; mRbAp48; NURF55; RBAP46; RBAP48; RBBP4; RBBP7
UniProt ID: (Human) Q09028, (Human) Q16576, (Mouse) Q60972, (Mouse) Q60973
Entrez Gene ID: (Human) 5928, (Human) 5931, (Mouse) 19646, (Mouse) 245688
negative regulation of transcription from RNA polymerase II promoter
DNA replication
transcription, DNA-templated
multicellular organism development
cell proliferation
histone deacetylation
negative regulation of cell growth
CENP-A containing nucleosome assembly
negative regulation of gene expression, epigenetic
response to steroid hormone
cellular heat acclimation
regulation of signal transduction by p53 class mediator
chromatin remodeling
regulation of transcription, DNA-templated
chromatin modification
negative regulation of transcription, DNA-templated
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
cell cycle
negative regulation of cell proliferation
chromatin assembly
ATP-dependent chromatin remodeling
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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