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Invitrogen
RbAp48 Monoclonal Antibody (9F3)
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RbAp48 Antibody (MA5-33003) in IHC (P)
Immunohistochemical analysis of RbAp48 in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-RbAp48 antibody (Product # MA5-33003) overnight at 4°C. Biotinylated goat anti-mou... View More
Product Details
MA5-33003
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
9F3
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RbAp48 (395-425aa EDNIMQVWQMAENIYNDEDPEGSVDPEGQGS).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2802637
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Jurkat whole cell, rat thymus tissue, mouse spleen tissue, human A549 whole cell, human Jurkat whole cell, human Hela whole cell, human PANC-1 whole cell. IHC: human intestinal cancer tissue, human placenta tissue, human mammary cancer tissue, human intestinal cancer tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue. Flow: SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAF-1 p48 subunit; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; Histone-binding protein RBBP4; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP48; RBBP-4; retinoblastoma binding protein 4; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48
Gene Aliases: lin-53; mRbAp48; NURF55; RBAP48; RBBP4
UniProt ID: (Human) Q09028, (Mouse) Q60972
Entrez Gene ID: (Human) 5928, (Rat) 313048, (Mouse) 19646
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
histone deacetylase activity
protein binding
DNA-dependent ATPase activity
nucleosomal DNA binding
histone binding
histone deacetylase binding
molecular_function
DNA replication-dependent nucleosome assembly
DNA replication-independent nucleosome assembly
chromatin remodeling
metabolic process
chromatin assembly
ATP-dependent chromatin remodeling
response to growth hormone
DNA replication
transcription, DNA-templated
regulation of transcription, DNA-templated
cell cycle
negative regulation of cell proliferation
histone deacetylation
CENP-A containing nucleosome assembly
negative regulation of gene expression, epigenetic
regulation of cell cycle
regulation of signal transduction by p53 class mediator
chromatin modification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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