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Invitrogen
RhoB Recombinant Rabbit Monoclonal Antibody (19H1L5)
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FIGURE: 1 / 2
RhoB Antibody (702242) in ICC/IF
For immunofluorescence analysis, A431 cells were fixed and permeabilized for detection of endogenous RhoB using Anti- RhoB Recombinant Rabbit Monoclonal Antibody (Product # 702242, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of RhoB protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)... View More
Product Details
702242
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
19H1L5
Immunogen
Peptides corresponding to Human RHOB (aa 128-145, 50-65)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2633072
Product Specific Information
This antibody is predicted to react with Monkey, Mouse, Goat and Cat
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Rho-related GTP-binding protein (RhoB) mediates apoptosis in neoplastically transformed cells after DNA damage. While not essential for development, it does affect cell adhesion and growth factor signaling in transformed cells. RhoB targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a tumor supressor - deletion of RhoB causes tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aplysia RAS-related homolog 6; h6; oncogene RHO H6; ras homolog gene family, member B; Rho; Rho cDNA clone 6; Rho-related GTP-binding protein RhoB
Gene Aliases: ARH6; ARHB; MST081; MSTP081; RHOB; RHOH6
UniProt ID: (Human) P62745
Entrez Gene ID: (Human) 388
cytokinesis
angiogenesis
intracellular protein transport
apoptotic process
cell adhesion
Rho protein signal transduction
endosome to lysosome transport
positive regulation of endothelial cell migration
cell differentiation
platelet activation
regulation of cell migration
negative regulation of cell migration
positive regulation of apoptotic process
positive regulation of angiogenesis
negative regulation of cell cycle
regulation of small GTPase mediated signal transduction
endothelial tube morphogenesis
cellular response to hydrogen peroxide
cellular response to ionizing radiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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