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Invitrogen
RhoF Polyclonal Antibody
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FIGURE: 1 / 1
RhoF Antibody (PA5-92293) in WB
Western blot analysis of RhoF in extracts of various cell lines. Samples were incubated with RhoF Polyclonal antibody (Product # PA5-92293) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-92293
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human RHOF (NP_0619072)
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806430
Product Specific Information
Immunogen sequence: SQGSFPEHYA PSVFEKYTAS VTVGSKEVTL NLYDTAGQED YDRLRPLSYQ NTHLVLICYD VMNPTSYDNV LIKWFPEVTH F; Positive Samples: HT-29, 293T, Mouse kidney; Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor, cytoskeleton
Target Information
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: induced in fatty liver dystrophy 1; ras homolog family member F (in filopodia); ras homolog gene family, member F (in filopodia); Rho family GTPase Rif; Rho in filopodia; Rho-related GTP-binding protein RhoF
Gene Aliases: AI845056; ARHF; AV026554; Ifld1; RHOF; RIF
UniProt ID: (Human) Q9HBH0, (Mouse) Q8BYP3
Entrez Gene ID: (Human) 54509, (Mouse) 23912
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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