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Invitrogen
RhoH Polyclonal Antibody
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FIGURE: 1 / 7
RhoH Antibody (PA5-99487) in ICC/IF
Immunofluorescent analysis of RhoH in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and RhoH polyclonal antibody (Product # PA5-99487) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-99487
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RHOH(Accession Q15669), corresponding to amino acid residues V114-E164.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818420
Product Specific Information
Antibody detects endogenous levels of total RhoH.
Target Information
The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTP-binding protein TTF; ras homolog gene family, member H; Rho-related GTP-binding protein RhoH; Translocation three four protein; TTF, translocation three four
Gene Aliases: 5830400A04Rik; ARHH; AU019774; RHOH; TTF
UniProt ID: (Human) Q15669, (Mouse) Q9D3G9
Entrez Gene ID: (Human) 399, (Mouse) 74734, (Rat) 305341
small GTPase mediated signal transduction
T cell differentiation
negative regulation of phosphorylation
negative regulation of catalytic activity
negative regulation of I-kappaB kinase/NF-kappaB signaling
mast cell activation
regulation of transcription, DNA-templated
negative regulation of GTPase activity
regulation of small GTPase mediated signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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