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Invitrogen
Rhodopsin Polyclonal Antibody
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FIGURE: 1 / 3
Rhodopsin Antibody (PA1-729) in WB
Western blot was performed using Anti-Rhodopsin Polyclonal Antibody (Product # PA1-729)and a 39 kDa band along with high molecular weight glycosylated forms corresponding to Rhodopsin were observed across all the tissues tested except Mouse Heart and Liver which are reported to be negative. Whole cell extracts (30 µg lysate) of Mouse Retina (Lane 1), Rat Retina (Lane 2), Rat Eye (Lane 3), Mouse Eye (Lane 4), Mouse Heart (Lane 5), and Mouse Liver (lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). R... View More
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.jpg?time=20250104?time=20250104 "Rhodopsin Antibody (PA1-729) in WB")
Product Details
PA1-729
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Sheep
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C S(338) K T E T S Q V A P A(348)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2178960
Product Specific Information
PA1-729 detects rhodopsin from bovine and sheep retina samples. This antibody does not detect rhodopsin from ovine samples
PA1-729 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.
The PA1-729 immunizing peptide (Cat. # PEP-174) corresponds to amino acid residues 338-348 from bovine rhodopsin. This peptide is available for use in neutralization and control experiments.
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC138309; MGC138311; OPN 2; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); RP 4
Gene Aliases: BOS_21149; RHO
UniProt ID: (Bovine) P02699
Entrez Gene ID: (Sheep) 101123241, (Bovine) 509933
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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