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Rif1 Antibody (600-401-871) in WB
Western blot using Rocklands Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 µg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilutio... View More
Product Details
600-401-871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 2375-2419 of Mouse Rif1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against mouse Rif1 protein. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with Rif1 from human, chimpanzee, rat and dog as only a single amino acid residue change (92% homology) is found for the immunogen sequence. Cross reactivity with Rif1 homologues from other sources has not been determined. Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa).
Target Information
May have a role in sperm development and fertilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp434D1026; DKFZp781N1478; FLJ12870; mRif1; Rap1-interacting factor 1 homolog; Telomere-associated protein RIF1
Gene Aliases: 5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145e; Rif1
UniProt ID: (Mouse) Q6PR54
Entrez Gene ID: (Mouse) 51869
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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