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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • S100 Antibodies

          Invitrogen

          S100 Monoclonal Antibody (4C4.9)

          3 Published Figures
          70 References
          View all (13) S100 antibodies

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          Datasheet
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          Cite S100 Monoclonal Antibody (4C4.9)

          • Antibody Testing Data (4)
          • Published Figures (3)
          S100 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          S100 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          S100 Antibody (MA5-12969) in ICC/IF

          Immunofluorescent analysis of S100 Protein (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a S100 Protein monoclonal antibody (Product # MA5-12969) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a D... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          S100 Antibody in Immunocytochemistry (ICC/IF)
          S100 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          S100 Antibody in Western Blot (WB)
          S100 Antibody in Western Blot (WB)
          S100 Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)
          S100 Antibody in Immunohistochemistry (IHC)
          S100 Antibody in Immunohistochemistry (IHC)

          Product Details

          MA5-12969

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:100-1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 56 publications 56 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse

          Published species

          Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Mouse / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4C4.9

          Immunogen

          Purified bovine brain S100 protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4, with 0.05% BSA

          Contains

          0.05% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10983883

          Product Specific Information

          MA5-12969 targets S100 Protein in IHC (P), ICC/IF and WB applications and shows reactivity with Bovine, Human, mouse, and Rat samples.

          The MA5-12969 immunogen is purified bovine brain S100 protein.

          Target Information

          S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Protein S100-A1; Protein S100-B; S 100; S-100 calcium-binding protein, beta chain; S-100 protein alpha chain; S-100 protein beta chain; S-100 protein subunit alpha; S-100 protein subunit beta; S100 alpha; S100 calcium-binding protein A1; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S100 protein, alpha polypeptide; S100 protein, beta polypeptide; S100alpha

          View more View less

          Gene Aliases: AI266795; AI850290; BOS_1447; BOS_2809; Bpb; NEF; S100; S100-alpha; S100-B; S100A; S100A1; S100B; S100beta

          View more View less

          UniProt ID: (Bovine) P02639, (Human) P23297, (Human) P04271, (Bovine) P02638, (Mouse) P56565, (Mouse) P50114

          View more View less

          Entrez Gene ID: (Bovine) 528735, (Human) 6271, (Human) 6285, (Bovine) 525716, (Mouse) 20193, (Mouse) 20203

          View more View less

          Function(s)
          calcium ion binding protein binding identical protein binding protein homodimerization activity S100 protein binding calcium-dependent protein binding ATPase binding zinc ion binding tau protein binding RAGE receptor binding kinase inhibitor activity metal ion binding receptor binding
          Process(es)
          axonogenesis central nervous system development learning or memory memory cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of neuronal synaptic plasticity astrocyte differentiation response to glucocorticoid response to methylmercury long-term synaptic potentiation cellular response to hypoxia negative regulation of skeletal muscle cell differentiation regulation of translation phosphorylation negative regulation of phosphorylation positive regulation of complement activation astrocyte activation energy reserve metabolic process cellular calcium ion homeostasis regulation of cytokine biosynthetic process regulation of long-term neuronal synaptic plasticity positive regulation of synaptic transmission negative regulation of transcription from RNA polymerase II promoter regulation of heart contraction substantia nigra development intracellular signal transduction positive regulation of voltage-gated calcium channel activity
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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