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Invitrogen
S100A10 Polyclonal Antibody
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FIGURE: 1 / 8
S100A10 Antibody (PA5-27251) in ICC/IF
Immunofluorescence analysis of Protein S100-A10 was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 15 minutes at room temperature. The cells were labeled with S100A10 Polyclonal Antibody (Product # PA5-27251) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Pr... View More
Product Details
PA5-27251
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human S100A10. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2544727
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa.
Predicted reactivity: Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The S-100 family of calcium-activated proteins interact with a range of target proteins to modulate biological signaling pathways. Numerous cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular cell surface, where it forms a heterotetrameric complex with Annexin II, though this association is not required for plasma membrane localization or binding and activation of plasminogen. Additionally, S-100A10 acts as a cellular chaperone for hepatitis B (Hep B) virus polymerase. Hep B virus polymerase normally localizes to the cytoplasm only, though in the presence of S-100A10 a portion relocates to the nucleus, implying a role for S-100A10 and intracellular calcium in the process of viral replication.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Annexin II; annexin II ligand; annexin II ligand, calpactin I, light polypeptide; annexin II tetramer (AIIt) p11 subunit; calcium binding protein A11 (calgizzarin); Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; MGC111133; MGC133268; Nerve growth factor-induced protein 42C; OTTHUMP00000015270; P PRSS26; p10 protein; p11; p11 subunit; Protein S100 A10; Protein S100-A10; S-100 related protein, clone 42C; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium binding protein A10 (calgizzarin); S100 calcium-binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11))
Gene Aliases: 42C; AA409961; AL024248; ANX2L; ANX2LG; Ca[1]; CAL12; CAL1L; CLP11; GP11; p10; P11; S100A10
UniProt ID: (Human) P60903, (Rat) P05943, (Mouse) P08207
Entrez Gene ID: (Human) 6281, (Rat) 81778, (Mouse) 20194
regulation of cell growth
membrane raft assembly
membrane budding
response to antidepressant
regulation of cell differentiation
positive regulation of binding
protein heterotetramerization
positive regulation of stress fiber assembly
positive regulation of focal adhesion assembly
establishment of protein localization to plasma membrane
positive regulation of substrate adhesion-dependent cell spreading
positive regulation of GTPase activity
cellular response to acid chemical
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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