Invitrogen

S100A8 Polyclonal Antibody

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S100A8 Antibody (PA5-79948) in WB

Western blot analysis of S100A8 using S100A8 Polyclonal Antibody (Product # PA5-79948). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat spleen tissue lysates. Lane 2: rat thymus tissue lysates. Lane 3: mouse spleen tissue lysates. Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked... View More

S100A8 Antibody in Flow Cytometry (Flow)

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Product Details

PA5-79948

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2747063

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue. IHC: rat spleen tissue, mouse spleen tissue. ICC/IF: NIH3T3 cell. Flow: HEPA 1-6 cell.

Target Information

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Salmonella pathogenicity island 1 knockdown confers protection against myocardial fibrosis and inflammation in uremic cardiomyopathy via down-regulation of S100 Calcium Binding Protein A8/A9 transcription.

Renal failure

Cai X,Hong L,Liu Y,Huang X,Lai H,Shao L

PA5-79948 was used in Western Blotting to dissect the mechanism of SPI1 in myocardial fibrosis and inflammation induced by UCM through S100A8/A9.

Thu Dec 01 00:00:00 UTC 2022

Renal failure

Cai X,Hong L,Liu Y,Huang X,Lai H,Shao L

PA5-79948 was used in Western Blotting to dissect the mechanism of SPI1 in myocardial fibrosis and inflammation induced by UCM through S100A8/A9.

Thu Dec 01 00:00:00 UTC 2022

S100A9 is a functional effector of infarct wall thinning after myocardial infarction.

American journal of physiology. Heart and circulatory physiology

Chalise U,Becirovic-Agic M,Daseke MJ,Konfrst SR,Rodriguez-Paar JR,Feng D,Salomon JD,Anderson DR,Cook LM,Lindsey ML

PA5-79948 was used in Western Blotting to determine protein changes over the course of neutrophil infiltration of ventricles following myocardial infarction in mice.

Tue Feb 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: Calgranulin-A; Chemotactic cytokine CP-10; chemotactic S100 protein; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A)

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Gene Aliases: 60B8Ag; AI323541; B8Ag; Caga; CFAg; CP-10; MRP8; p8; S100a8

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UniProt ID: (Rat) P50115, (Mouse) P27005

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Entrez Gene ID: (Rat) 116547, (Mouse) 20201

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Function(s)

molecular_function calcium ion binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding metal ion binding

Process(es)

leukocyte migration involved in inflammatory response acute inflammatory response chronic inflammatory response positive regulation of peptide secretion autophagy activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response response to zinc ion neutrophil chemotaxis response to lipopolysaccharide wound healing innate immune response response to ethanol positive regulation of inflammatory response neutrophil aggregation positive regulation of intrinsic apoptotic signaling pathway immune system process apoptotic process chemotaxis peptidyl-cysteine S-nitrosylation regulation of inflammatory response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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S100A8 Polyclonal Antibody

S100A8 Antibody (PA5-79948) in WB

Product Details

PA5-79948

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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