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Invitrogen
S100A8 Monoclonal Antibody (335806)
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FIGURE: 1 / 2
S100A8 Antibody (MA5-24066) in ICC/IF
Immunocytochemistry analysis of S100A8 in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line. Samples were incubated in S100A8 monoclonal antibody (Product # MA5-24066) using a dilution of 10 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue).
Product Details
MA5-24066
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
335806
Immunogen
E. coli-derived recombinant mouse S100A8 Met1-Glu89 (predicted)
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609086
Product Specific Information
In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calgranulin-A; Chemotactic cytokine CP-10; chemotactic S100 protein; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 A8; S100 calcium-binding protein A8
Gene Aliases: 60B8Ag; AI323541; B8Ag; Caga; CFAg; CP-10; MRP8; p8; S100a8
UniProt ID: (Mouse) P27005
Entrez Gene ID: (Mouse) 20201
immune system process
leukocyte migration involved in inflammatory response
positive regulation of peptide secretion
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
chemotaxis
inflammatory response
peptidyl-cysteine S-nitrosylation
neutrophil chemotaxis
innate immune response
regulation of inflammatory response
positive regulation of inflammatory response
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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