Invitrogen

S100A9 Polyclonal Antibody

S100A9 Antibody in Immunocytochemistry (ICC/IF)

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S100A9 Antibody (PA1-46489) in ICC/IF

Immunofluorescence analysis of S100A9 was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with S100A9 Polyclonal Antibody (Product # PA1-46489) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at... View More

S100A9 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-46489

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human S100A9 protein expressed in E. coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10977234

Product Specific Information

Suggested positive control: antigen standard for S100A9 (transient overexpression lysate), HL-60 + DMSO.

Target Information

S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization.

Communications biology

Chen YC,Smith M,Ying YL,Makridakis M,Noonan J,Kanellakis P,Rai A,Salim A,Murphy A,Bobik A,Vlahou A,Greening DW,Peter K

PA1-46489 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to provide a knowledge resource defining unstable atherosclerosis that will facilitate the identification and validation of long-sought-after therapeutic targets and drugs for plaque stabilization.

Mon Mar 13 00:00:00 UTC 2023

Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization.

Communications biology

Chen YC,Smith M,Ying YL,Makridakis M,Noonan J,Kanellakis P,Rai A,Salim A,Murphy A,Bobik A,Vlahou A,Greening DW,Peter K

Mon Mar 13 00:00:00 UTC 2023

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.

The Journal of cell biology

Stempels FC,Jiang M,Warner HM,Moser ML,Janssens MH,Maassen S,Nelen IH,de Boer R,Jiemy WF,Knight D,Selley J,O'Cualain R,Baranov MV,Burgers TCQ,Sansevrino R,Milovanovic D,Heeringa P,Jones MC,Vlijm R,Ter Beest M,van den Bogaart G

PA1-46489 was used in Immunocytochemistry to propose that the ESCRT proteins are recruited to adhesion-induced membrane tears to induce extracellular shedding of the damaged membrane.

Mon Jul 03 00:00:00 UTC 2023

Rapamycin-filgrastim combination therapy ameliorates portal hypertension-induced splenomegaly: Role of β actin and S100A9 proteins modulation.

Iranian journal of basic medical sciences

Abdelrahman SA,Abdelfatah MM,Keshta AT

PA1-46489 was used in Immunohistochemistry to assess the mechanisms underlying the effects of rapamycin and filgrastim when taken separately or in combination on the biochemical and histopathological aspects of the liver and spleen.

Wed Jun 01 00:00:00 UTC 2022

Rapamycin-filgrastim combination therapy ameliorates portal hypertension-induced splenomegaly: Role of β actin and S100A9 proteins modulation.

Iranian journal of basic medical sciences

Abdelrahman SA,Abdelfatah MM,Keshta AT

Wed Jun 01 00:00:00 UTC 2022

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Aging

Xu Z,Cheng C,Kong R,Liu Y,Wang S,Ma Y,Xing X

PA1-46489 was used in Immunocytochemistry to find that S100A8 and S100A9 were both upregulated in burn-wounded skins in vivo and thermal-stimulated epidermal keratinocytes in vitro, accompanied by increased levels of epithelial-mesenchymal transition.

Mon Jun 07 00:00:00 UTC 2021

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Aging

Xu Z,Cheng C,Kong R,Liu Y,Wang S,Ma Y,Xing X

Mon Jun 07 00:00:00 UTC 2021

Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.

Molecular medicine reports

Tan N,Hu S,Hu Z,Wu Z,Wang B

PA1-46489 was used in Western Blotting to identify differentially expressed proteins (DEPs) in microvesicles/exosomes (MV/E) isolated from the cerebrospinal fluid (CSF) of patients with ABE.

Sat Aug 01 00:00:00 UTC 2020

Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.

Molecular medicine reports

Tan N,Hu S,Hu Z,Wu Z,Wang B

PA1-46489 was used in Western Blotting to identify differentially expressed proteins (DEPs) in microvesicles/exosomes (MV/E) isolated from the cerebrospinal fluid (CSF) of patients with ABE.

Sat Aug 01 00:00:00 UTC 2020

Distinct molecular signatures of mild extrinsic and intrinsic atopic dermatitis.

Experimental dermatology

Martel BC,Litman T,Hald A,Norsgaard H,Lovato P,Dyring-Andersen B,Skov L,Thestrup-Pedersen K,Skov S,Skak K,Poulsen LK

PA1-46489 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to identify differentially expressed genes involved in skin barrier formation and inflammation, and to compare our results with those reported for patients with moderate and severe AD.

Wed Jun 01 00:00:00 UTC 2016

Distinct molecular signatures of mild extrinsic and intrinsic atopic dermatitis.

Experimental dermatology

Martel BC,Litman T,Hald A,Norsgaard H,Lovato P,Dyring-Andersen B,Skov L,Thestrup-Pedersen K,Skov S,Skak K,Poulsen LK

Wed Jun 01 00:00:00 UTC 2016

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Proteome science

Monari E,Cuoghi A,Bellei E,Bergamini S,Lucchi A,Tomasi A,Cortellini P,Zaffe D,Bertoldi C

PA1-46489 was used in western blot to determine the proteomic profile of periodontally-affected patients

Thu Dec 31 00:00:00 UTC 2015

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Proteome science

Monari E,Cuoghi A,Bellei E,Bergamini S,Lucchi A,Tomasi A,Cortellini P,Zaffe D,Bertoldi C

PA1-46489 was used in western blot to determine the proteomic profile of periodontally-affected patients

Thu Dec 31 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Myeloid-related protein 14; p14; Protein S100-A9; S100 A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)

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Gene Aliases: 60B8AG; AW546964; BEE22; CAGB; CFAG; CGLB; GAGB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9

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UniProt ID: (Human) P06702, (Mouse) P31725, (Rat) P50116

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Entrez Gene ID: (Human) 6280, (Mouse) 20202, (Rat) 94195

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Function(s)

signal transducer activity calcium ion binding protein binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding metal ion binding molecular_function

Process(es)

immune system process leukocyte migration involved in inflammatory response positive regulation of peptide secretion regulation of translation autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process chemotaxis inflammatory response peptidyl-cysteine S-nitrosylation neutrophil chemotaxis leukocyte chemotaxis actin cytoskeleton reorganization peptidyl-cysteine S-trans-nitrosylation innate immune response regulation of integrin biosynthetic process regulation of inflammatory response positive regulation of inflammatory response neutrophil aggregation positive regulation of intrinsic apoptotic signaling pathway chronic inflammatory response response to zinc ion response to lipopolysaccharide response to ethanol cytokine production signal transduction cell-cell signaling astrocyte development positive regulation of blood coagulation positive regulation of cell growth sequestering of zinc ion chemokine production defense response to bacterium defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization cellular oxidant detoxification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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S100A9 Polyclonal Antibody

S100A9 Antibody (PA1-46489) in ICC/IF

Product Details

PA1-46489

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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