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Invitrogen
S100A9 Polyclonal Antibody
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FIGURE: 1 / 7
S100A9 Antibody (PA5-79950) in ICC/IF
Immunocytochemistry analysis of S100A9 using anti-S100A9 antibody (Product # PA5-79950) . S100A9 was detected in a section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 5μg/mL rabbit anti-S100A9 antibody (Product # PA5-79950) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with ... View More
Product Details
PA5-79950
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747065
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: mouse spleen tissue, mouse lung tissue. IHC: mouse spleen tissue, rat spleen tissue. ICC/IF: HEPA1-6 cell. Flow: HEPA1-6 cell.
Target Information
S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Myeloid-related protein 14; p14; Protein S100-A9; S100 A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)
Gene Aliases: 60B8AG; AW546964; BEE22; CAGB; CFAG; CGLB; GAGB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9
UniProt ID: (Human) P06702, (Mouse) P31725, (Rat) P50116
Entrez Gene ID: (Human) 6280, (Mouse) 20202, (Rat) 94195
immune system process
leukocyte migration involved in inflammatory response
positive regulation of peptide secretion
regulation of translation
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
chemotaxis
inflammatory response
peptidyl-cysteine S-nitrosylation
neutrophil chemotaxis
leukocyte chemotaxis
actin cytoskeleton reorganization
peptidyl-cysteine S-trans-nitrosylation
innate immune response
regulation of integrin biosynthetic process
regulation of inflammatory response
positive regulation of inflammatory response
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
chronic inflammatory response
response to zinc ion
response to lipopolysaccharide
response to ethanol
cytokine production
signal transduction
cell-cell signaling
astrocyte development
positive regulation of blood coagulation
positive regulation of cell growth
sequestering of zinc ion
chemokine production
defense response to bacterium
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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