Invitrogen

S100B Polyclonal Antibody

S100B Antibody in Immunocytochemistry (ICC/IF)

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S100B Antibody (PA5-78161) in ICC/IF

S100B Polyclonal Antibody detects S100 beta protein at glia cells by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: S100 beta stained by S100B Polyclonal Antibody (Product # PA5-78161) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with DAPI .

S100B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S100B Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

S100B Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of Protein S100-B was observed higher in mouse brain, rat brain, mouse cerebellum and rat cerebellum as compared to mouse heart and rat heart using Anti-S100B Polyclonal Antibody (Product # PA5-78161) in Western Blot. {RE}

Product Details

PA5-78161

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rodent, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human S100 beta Recombinant protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2736549

Product Specific Information

Positive Control: V5-human S100B-transfected 293T, A375, mouse brain, rat brain

Predicted Reactivity: Rabbit (100%), Chicken (84%), Rhesus Monkey (98%), Bovine (96%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recording plasticity in neuronal activity in the rodent intrinsic cardiac nervous system using calcium imaging techniques.

Frontiers in synaptic neuroscience

Smith JEG,Ashton JL,Argent LP,Cheyne JE,Montgomery JM

PA5-78161 was used in Immunohistochemistry to provide evidence for a potential link between arrhythmia and plasticity in calcium dynamics that occur not only in cardiomyocytes but also in the GP neurons of the heart.

Sat Apr 22 00:00:00 UTC 2023

Recording plasticity in neuronal activity in the rodent intrinsic cardiac nervous system using calcium imaging techniques.

Frontiers in synaptic neuroscience

Smith JEG,Ashton JL,Argent LP,Cheyne JE,Montgomery JM

Sat Apr 22 00:00:00 UTC 2023

Novel patient-derived xenograft and cell line models for therapeutic screening in NF2-associated schwannoma.

The Journal of pathology

Zhao F,Chen Y,Li SW,Zhang J,Zhang S,Zhao XB,Yang ZJ,Wang B,He QY,Wang LM,Xu L,Liu PN

Published figure using S100B polyclonal antibody (Product # PA5-78161) in Immunocytochemistry

Mon Aug 01 00:00:00 UTC 2022

Oligodendrocytes are susceptible to Zika virus infection in a mouse model of perinatal exposure: Implications for CNS complications.

Glia

Schultz V,Barrie JA,Donald CL,Crawford CL,Mullin M,Anderson TJ,Solomon T,Barnett SC,Linington C,Kohl A,Willison HJ,Edgar JM

PA5-78161 was used in Immunohistochemistry to demonstrate that a clinically relevant ZIKV isolate can directly impact oligodendrocytes.

Sun Aug 01 00:00:00 UTC 2021

Murine muscle stem cell response to perturbations of the neuromuscular junction are attenuated with aging.

eLife

Larouche JA,Mohiuddin M,Choi JJ,Ulintz PJ,Fraczek P,Sabin K,Pitchiaya S,Kurpiers SJ,Castor-Macias J,Liu W,Hastings RL,Brown LA,Markworth JF,De Silva K,Levi B,Merajver SD,Valdez G,Chakkalakal JV,Jang YC,Brooks SV,Aguilar CA

PA5-78161 was used in Immunocytochemistry to investigate if unique features of murine muscle stem cells exist that respond to synaptic perturbations caused by aging and other stressors.

Thu Jul 29 00:00:00 UTC 2021

Murine muscle stem cell response to perturbations of the neuromuscular junction are attenuated with aging.

eLife

PA5-78161 was used in Immunocytochemistry to investigate if unique features of murine muscle stem cells exist that respond to synaptic perturbations caused by aging and other stressors.

Thu Jul 29 00:00:00 UTC 2021

Astrocytes Exhibit a Protective Role in Neuronal Firing Patterns under Chemically Induced Seizures in Neuron-Astrocyte Co-Cultures.

International journal of molecular sciences

Ahtiainen A,Genocchi B,Tanskanen JMA,Barros MT,Hyttinen JAK,Lenk K

PA5-78161 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to assess the modulatory roles of astrocytes during epileptic-like conditions and in compensating drug-elicited hyperactivity.

Thu Nov 25 00:00:00 UTC 2021

Vitamin D₃ and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti-inflammatory actions by remodeling cellular calcium pathways.

Journal of biochemical and molecular toxicology

El-Boshy M,Refaat B,Almaimani RA,Abdelghany AH,Ahmad J,Idris S,Almasmoum H,Mahbub AA,Ghaith MM,BaSalamah MA

PA5-78161 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to show the protective effects of vitamin D calcium supplements against cadmium hepatotoxicity.

Sun Mar 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein beta chain; S100 protein, beta polypeptide, neural

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Gene Aliases: AI850290; Bpb; NEF; S100; S100-B; S100B; S100beta; S100P

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UniProt ID: (Human) P04271, (Mouse) P50114, (Rat) P04631

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Entrez Gene ID: (Human) 6285, (Mouse) 20203, (Rat) 25742

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Function(s)

calcium ion binding protein binding zinc ion binding identical protein binding protein homodimerization activity S100 protein binding tau protein binding calcium-dependent protein binding RAGE receptor binding receptor binding metal ion binding

Process(es)

axonogenesis central nervous system development learning or memory memory cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of neuronal synaptic plasticity astrocyte differentiation response to glucocorticoid response to methylmercury long-term synaptic potentiation cellular response to hypoxia negative regulation of skeletal muscle cell differentiation energy reserve metabolic process cellular calcium ion homeostasis regulation of cytokine biosynthetic process regulation of long-term neuronal synaptic plasticity positive regulation of synaptic transmission

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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S100B Polyclonal Antibody

S100B Antibody (PA5-78161) in ICC/IF

Product Details

PA5-78161

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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