Invitrogen

S100B Recombinant Rabbit Monoclonal Antibody (SC57-02)

S100B Antibody in Immunocytochemistry (ICC/IF)

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S100B Antibody (MA5-42438) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of S100 beta in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with S100B Recombinant Monoclonal Antibody (Product # MA5-42438) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (... View More

S100B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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S100B Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of S100B was observed in SK-MEL-28 which is reported to be positive but not in T-47D. Similarly in mouse tissues, the expression of S100B was observed in mouse brain and mouse cerebellum which is reported to be positive but not in mouse heart using Anti-S100B Recombinant Rabbit Monoclonal Antibody (SC57-02) (Product # MA5-42438) in western blot. {RE}

Product Details

MA5-42438

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Goat, Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC57-02

Immunogen

Synthetic peptide within C-terminal human S100 beta

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2911579

Product Specific Information

Positive Control: Mouse liver tissue lysates, zebrafish tissue lysates, Hela, N2A, SH-SY5Y, mouse brain tissue.

Subcellular Location: Cytoplasm, Nucleus.

Target Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Protein S100-B; S-100 calcium-binding protein, beta chain; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium binding protein beta (neural); S100 calcium-binding protein B; S100 calcium-binding protein beta (neural); S100 calcium-binding protein, beta (neural); S100 protein beta chain; S100 protein, beta polypeptide, neural

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Gene Aliases: AI850290; Bpb; NEF; S100; S100-B; S100B; S100beta; S100P; si:dkey-110p14.2; wu:fq18b10; zgc:92776

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UniProt ID: (Human) P04271, (Mouse) P50114, (Rat) P04631

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Entrez Gene ID: (Human) 6285, (Mouse) 20203, (Rat) 25742, (Zebrafish) 436825

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Function(s)

calcium ion binding protein binding zinc ion binding identical protein binding protein homodimerization activity S100 protein binding tau protein binding calcium-dependent protein binding RAGE receptor binding receptor binding metal ion binding

Process(es)

axonogenesis central nervous system development learning or memory memory cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of neuronal synaptic plasticity astrocyte differentiation response to glucocorticoid response to methylmercury long-term synaptic potentiation cellular response to hypoxia negative regulation of skeletal muscle cell differentiation energy reserve metabolic process cellular calcium ion homeostasis regulation of cytokine biosynthetic process regulation of long-term neuronal synaptic plasticity positive regulation of synaptic transmission

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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S100B Recombinant Rabbit Monoclonal Antibody (SC57-02)

S100B Antibody (MA5-42438) in ICC/IF

Product Details

MA5-42438

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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