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S1PR1/EDG1 Polyclonal Antibody

View all (27) S1P1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (6)

S1PR1/EDG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S1PR1/EDG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

S1PR1/EDG1 Antibody (55133-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 55133-1-AP ( S1PR1/EDG1 antibody at dilution of 1:100 (under 40x lens).

S1PR1/EDG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S1PR1/EDG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S1PR1/EDG1 Antibody in Western Blot (WB)

S1PR1/EDG1 Antibody in Western Blot (WB)

S1PR1/EDG1 Antibody in Western Blot (WB)

S1PR1/EDG1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

S1PR1/EDG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 55133-1-AP ( S1PR1/EDG1 antibody at dilution of 1:100 (under 40x lens).

S1PR1/EDG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human placenta tissue slide using 55133-1-AP ( S1PR1/EDG1 antibody at dilution of 1:100 (under 10x lens).

S1PR1/EDG1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 55133-1-AP) S1PR1/EDG1 antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

S1PR1/EDG1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 55133-1-AP (S1PR1/EDG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

S1PR1/EDG1 Antibody in Western Blot (WB) — SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55133-1-AP (S1PR1/EDG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

S1PR1/EDG1 Antibody in Immunoprecipitation (IP) — IP result of anti-S1PR1/EDG1 (IP:55133-1-AP, 4ug; Detection:55133-1-AP 1:300) with mouse brain tissue lysate 2640ug.

Product Details

55133-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:600

View 19 publications 19 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

-

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

-

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.77 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M sodium bicarbonate/0.1M glycine, pH 7.3, with 50% glycerol, 0.1M glycine

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to S1PR1.

Target Information

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dexmedetomidine alleviates oxidative stress and mitochondrial dysfunction in diabetic peripheral neuropathy via the microRNA-34a/SIRT2/S1PR1 axis.

International immunopharmacology

Lin Y,Wei Y,Wei Y,Yu H,Zhang W,Li C,He Y,Yao G,Zhang Y

Sat Apr 01 00:00:00 UTC 2023

A novel co-target of ACY1 governing plasma membrane translocation of SphK1 contributes to inflammatory and neuropathic pain.

iScience

Liu B,Wu W,Cui L,Zheng X,Li N,Zhang X,Duan G

Fri Jun 16 00:00:00 UTC 2023

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Acta pharmaceutica Sinica. B

Hao M,Fu R,Tai J,Tian Z,Yuan X,Chen Y,Wang M,Jiang H,Ji M,Lai F,Xue N,Bai L,Zhu Y,Lv X,Chen X,Jin J

Wed Mar 01 00:00:00 UTC 2023

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Acta pharmaceutica Sinica. B

Hao M,Fu R,Tai J,Tian Z,Yuan X,Chen Y,Wang M,Jiang H,Ji M,Lai F,Xue N,Bai L,Zhu Y,Lv X,Chen X,Jin J

Wed Mar 01 00:00:00 UTC 2023

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Acta pharmaceutica Sinica. B

Hao M,Fu R,Tai J,Tian Z,Yuan X,Chen Y,Wang M,Jiang H,Ji M,Lai F,Xue N,Bai L,Zhu Y,Lv X,Chen X,Jin J

Wed Mar 01 00:00:00 UTC 2023

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Acta pharmaceutica Sinica. B

Hao M,Fu R,Tai J,Tian Z,Yuan X,Chen Y,Wang M,Jiang H,Ji M,Lai F,Xue N,Bai L,Zhu Y,Lv X,Chen X,Jin J

Wed Mar 01 00:00:00 UTC 2023

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Acta pharmaceutica Sinica. B

Hao M,Fu R,Tai J,Tian Z,Yuan X,Chen Y,Wang M,Jiang H,Ji M,Lai F,Xue N,Bai L,Zhu Y,Lv X,Chen X,Jin J

Wed Mar 01 00:00:00 UTC 2023

S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung.

Acta pharmaceutica Sinica. B

Hao M,Fu R,Tai J,Tian Z,Yuan X,Chen Y,Wang M,Jiang H,Ji M,Lai F,Xue N,Bai L,Zhu Y,Lv X,Chen X,Jin J

Wed Mar 01 00:00:00 UTC 2023

Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke.

iScience

Fukuzaki Y,Faustino J,Lecuyer M,Rayasam A,Vexler ZS

Fri Apr 21 00:00:00 UTC 2023

Sphingosine 1-phosphate receptor 1 regulates blood-brain barrier permeability in epileptic mice.

Neural regeneration research

Yang LX,Yao YY,Yang JR,Cheng HL,Zhu XJ,Zhang ZJ

Tue Aug 01 00:00:00 UTC 2023

Sphingosine 1-phosphate receptor 1 regulates blood-brain barrier permeability in epileptic mice.

Neural regeneration research

Yang LX,Yao YY,Yang JR,Cheng HL,Zhu XJ,Zhang ZJ

Tue Aug 01 00:00:00 UTC 2023

Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis.

Cell reports

Janneh AH,Kassir MF,Atilgan FC,Lee HG,Sheridan M,Oleinik N,Szulc Z,Voelkel-Johnson C,Nguyen H,Li H,Peterson YK,Marangoni E,Saatci O,Sahin O,Lilly M,Atkinson C,Tomlinson S,Mehrotra S,Ogretmen B

Tue Dec 06 00:00:00 UTC 2022

Potential of a sphingosine 1-phosphate receptor antagonist and sphingosine kinase inhibitors as targets for multiple myeloma treatment.

Oncology letters

Tanaka Y,Okabe S,Ohyashiki K,Gotoh A

Fri Apr 01 00:00:00 UTC 2022

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wang Y,Wu H,Gui BJ,Liu J,Rong GX,Deng R,Bu YH,Zhang H

Wed Jun 01 00:00:00 UTC 2022

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wang Y,Wu H,Gui BJ,Liu J,Rong GX,Deng R,Bu YH,Zhang H

Wed Jun 01 00:00:00 UTC 2022

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wang Y,Wu H,Gui BJ,Liu J,Rong GX,Deng R,Bu YH,Zhang H

Wed Jun 01 00:00:00 UTC 2022

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Wang Y,Wu H,Gui BJ,Liu J,Rong GX,Deng R,Bu YH,Zhang H

Wed Jun 01 00:00:00 UTC 2022

Interference with the expression of S1PR1 or STAT3 attenuates valvular damage due to rheumatic heart disease.

International journal of molecular medicine

Xian S,Chen A,Wu Y,Wen H,Lu C,Huang F,Zeng Z

Wed Sep 01 00:00:00 UTC 2021

Bone marrow mesenchymal stem cells-derived exosomes reduce Aβ deposition and improve cognitive function recovery in mice with Alzheimer's disease by activating sphingosine kinase/sphingosine-1-phosphate signaling pathway.

Cell biology international

Wang X,Yang G

Thu Apr 01 00:00:00 UTC 2021

Inhibition of sphingosine 1-phosphate (S1P) receptor 1/2/3 ameliorates biological dysfunction in rheumatoid arthritis fibroblast-like synoviocyte MH7A cells through Gαi/Gαs rebalancing.

Clinical and experimental pharmacology & physiology

Wang M,Wu H,Wang R,Dai X,Deng R,Wang Y,Bu Y,Sun M,Zhang H

Sun Aug 01 00:00:00 UTC 2021

Inhibition of sphingosine 1-phosphate (S1P) receptor 1/2/3 ameliorates biological dysfunction in rheumatoid arthritis fibroblast-like synoviocyte MH7A cells through Gαi/Gαs rebalancing.

Clinical and experimental pharmacology & physiology

Wang M,Wu H,Wang R,Dai X,Deng R,Wang Y,Bu Y,Sun M,Zhang H

Sun Aug 01 00:00:00 UTC 2021

Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture.

Frontiers in cardiovascular medicine

Pan G,Liao M,Dai Y,Li Y,Yan X,Mai W,Liu J,Liao Y,Qiu Z,Zhou Z

Tue Jan 04 00:00:00 UTC 2022

Inhibition of Sphingosine-1-Phosphate Receptor 2 Prevents Thoracic Aortic Dissection and Rupture.

Frontiers in cardiovascular medicine

Pan G,Liao M,Dai Y,Li Y,Yan X,Mai W,Liu J,Liao Y,Qiu Z,Zhou Z

Tue Jan 04 00:00:00 UTC 2022

Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.

Phytotherapy research : PTR

Wang Y,Wu H,Deng R,Dai XJ,Bu YH,Sun MH,Zhang H,Wang MD,Wang RH

Sun Aug 01 00:00:00 UTC 2021

The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.

Frontiers in cell and developmental biology

Yeon M,Kim Y,Pathak D,Kwon E,Kim DY,Jeong MS,Jung HS,Jeoung D

Sat Jun 12 00:00:00 UTC 2021

The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.

Frontiers in cell and developmental biology

Yeon M,Kim Y,Pathak D,Kwon E,Kim DY,Jeong MS,Jung HS,Jeoung D

Sat Jun 12 00:00:00 UTC 2021

The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.

Frontiers in cell and developmental biology

Yeon M,Kim Y,Pathak D,Kwon E,Kim DY,Jeong MS,Jung HS,Jeoung D

Sat Jun 12 00:00:00 UTC 2021

Coix Seed Oil Exerts an Anti-Triple-Negative Breast Cancer Effect by Disrupting miR-205/S1PR1 Axis.

Frontiers in pharmacology

Fang T,Jiang YX,Chen L,Huang L,Tian XH,Zhou YD,Nagle DG,Zhang DD

Wed Oct 28 00:00:00 UTC 2020

S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway.

International immunopharmacology

Zheng Z,Zeng YZ,Ren K,Zhu X,Tan Y,Li Y,Li Q,Yi GH

Tue Jan 01 00:00:00 UTC 2019

S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway.

International immunopharmacology

Zheng Z,Zeng YZ,Ren K,Zhu X,Tan Y,Li Y,Li Q,Yi GH

Tue Jan 01 00:00:00 UTC 2019

Combination of sphingosine-1-phosphate receptor 1 (S1PR1) agonist and antiviral drug: a potential therapy against pathogenic influenza virus.

Scientific reports

Zhao J,Zhu M,Jiang H,Shen S,Su X,Shi Y

Wed Mar 27 00:00:00 UTC 2019

Combination of sphingosine-1-phosphate receptor 1 (S1PR1) agonist and antiviral drug: a potential therapy against pathogenic influenza virus.

Scientific reports

Zhao J,Zhu M,Jiang H,Shen S,Su X,Shi Y

Wed Mar 27 00:00:00 UTC 2019

Hepatocyte-Derived Lipotoxic Extracellular Vesicle Sphingosine 1-Phosphate Induces Macrophage Chemotaxis.

Frontiers in immunology

Liao CY,Song MJ,Gao Y,Mauer AS,Revzin A,Malhi H

Fri Oct 25 00:00:00 UTC 2019

Hepatocyte-Derived Lipotoxic Extracellular Vesicle Sphingosine 1-Phosphate Induces Macrophage Chemotaxis.

Frontiers in immunology

Liao CY,Song MJ,Gao Y,Mauer AS,Revzin A,Malhi H

Fri Oct 25 00:00:00 UTC 2019

Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.

PloS one

Matsushima-Nishiwaki R,Yamada N,Fukuchi K,Kozawa O

Fri May 10 00:00:00 UTC 2019

Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.

PloS one

Matsushima-Nishiwaki R,Yamada N,Fukuchi K,Kozawa O

Fri May 10 00:00:00 UTC 2019

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Frontiers in pharmacology

Pang HQ,Yue SJ,Tang YP,Chen YY,Tan YJ,Cao YJ,Shi XQ,Zhou GS,Kang A,Huang SL,Shi YJ,Sun J,Tang ZS,Duan JA

Thu Oct 01 00:00:00 UTC 2020

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Frontiers in pharmacology

Pang HQ,Yue SJ,Tang YP,Chen YY,Tan YJ,Cao YJ,Shi XQ,Zhou GS,Kang A,Huang SL,Shi YJ,Sun J,Tang ZS,Duan JA

Thu Oct 01 00:00:00 UTC 2020

Sphingosine-1-phosphate mediates AKT/ERK maintenance of dental pulp homoeostasis.

International endodontic journal

Pan HY,Yang H,Shao MY,Xu J,Zhang P,Cheng R,Hu T

Fri May 01 00:00:00 UTC 2015

Sphingosine-1-phosphate mediates AKT/ERK maintenance of dental pulp homoeostasis.

International endodontic journal

Pan HY,Yang H,Shao MY,Xu J,Zhang P,Cheng R,Hu T

Fri May 01 00:00:00 UTC 2015

H9N2 avian influenza infection altered expression pattern of sphiogosine-1-phosphate receptor 1 in BALB/c mice.

Virology journal

Tong S,Tian J,Wang H,Huang Z,Yu M,Sun L,Liu R,Liao M,Ning Z

Mon Sep 30 00:00:00 UTC 2013

H9N2 avian influenza infection altered expression pattern of sphiogosine-1-phosphate receptor 1 in BALB/c mice.

Virology journal

Tong S,Tian J,Wang H,Huang Z,Yu M,Sun L,Liu R,Liao M,Ning Z

Mon Sep 30 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD antigen CD363; CD363; CD363 antigen; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; Lysophospholipid receptor B1; S1P receptor 1; S1P receptor Edg-1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

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Gene Aliases: AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Lpb1; S1p; S1P1; S1PR1

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UniProt ID: (Human) P21453, (Mouse) O08530

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Entrez Gene ID: (Human) 1901, (Mouse) 13609

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G-protein coupled receptor binding G-protein coupled receptor activity sphingosine-1-phosphate receptor activity sphingolipid binding signal transducer activity

angiogenesis blood vessel maturation cardiac muscle tissue growth involved in heart morphogenesis sphingosine-1-phosphate signaling pathway chemotaxis cell adhesion G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway brain development cell migration transmission of nerve impulse lamellipodium assembly regulation of cell adhesion neuron differentiation positive regulation of cell migration regulation of bone mineralization actin cytoskeleton reorganization positive regulation of GTPase activity regulation of bone resorption endothelial cell differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle cell proliferation positive regulation of positive chemotaxis positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of stress fiber assembly heart trabecula morphogenesis T cell migration signal transduction positive regulation of cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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