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Invitrogen
SAP (SLAM-Associated Protein) Monoclonal Antibody (10C4.2), eBioscience™
-WB-20170216130641.jpg?time=20250104 "SAP (SLAM-Associated Protein) Antibody in Western Blot (WB)")
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-WB-20170216130641.jpg?time=20250104?time=20250104 "SAP (SLAM-Associated Protein) Antibody (14-9888-82) in WB")
Product Details
14-9888-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
10C4.2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10667746
Product Specific Information
Description: This 10C4.2 monoclonal antibody reacts with human SLAM-associated protein (SAP). Upon SLAM receptor ligation, this 16-kDa SH2 domain-containing adaptor protein recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events. SAP is expressed in T, NK, and NKT cells, as well as eosinophils and platelets. This protein has also been detected in B cells and tumor cell lines such as Jurkat and Raji. Studies demonstrate that SAP expression in T follicular helper cells is required for germinal center formation. Moreover, this protein is involved in NKT cell development, Th1/Th2 differentiation, and controlling NK cytotoxicity. The role of SAP in B cells, however, is currently under investigation. Finally, deletion of the gene encoding SAP, SH2D1A, results in X-linked lymphoproliferative disease in humans.
Applications Reported: This 10C4-SAP (10C4.2) antibody has been reported for use in immunoblotting and immunocytochemistry (ICC).
Applications Tested: This 10C4.2 antibody has been tested by immunoblotting of reduced cell lysate prepared from Jurkat cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Duncan disease SH2-protein; Duncan's disease; FLJ18687; FLJ92177; HGNC:10820; RP5-1052M9.3; SH2 domain-containing protein 1A; signaling lymphocyte activation molecule-associated protein; Signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP
Gene Aliases: DSHP; EBVS; IMD5; LYP; MTCP1; SAP; SAP/SH2D1A; SH2D1A; XLP; XLPD; XLPD1
UniProt ID: (Human) O60880
Entrez Gene ID: (Human) 4068
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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