Invitrogen

SAP97 Polyclonal Antibody

SAP97 Antibody in Immunocytochemistry (ICC/IF)

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SAP97 Antibody (PA1-741) in ICC/IF

Immunofluorescence analysis of SAP97 was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SAP97 Rabbit Polyclonal Antibody (Product # PA1-741) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More

SAP97 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

SAP97 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

SAP97 Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

SAP97 Antibody in Immunohistochemistry - Free Floating (IHC (Free))

SAP97 Antibody in Western Blot, Immunoprecipitation (WB, IP)

SAP97 Antibody in Immunoprecipitation (IP)

Product Image Gallery

SAP97 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-SAP97 Polyclonal Antibody (Product # PA1-741), shows induced expression of the target protein in differentiated neural stem cells (NSC) as compared to undifferentiated NSCs. {RE}

Product Details

PA1-741

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:200 - 1:250

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues V(115) L P S E R I S P Q V P N E V L G P E(133) of rat SAP97.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2092020

Product Specific Information

PA1-741 detects Synapse-Associated Protein 97 (SAP97) from human, rat and mouse tissues. No cross-reactivity to other proteins, including synapse-associated protein family members, has been observed.

PA1-741 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunoprecipitation, and immunohistochemistry procedures. By Western blot, this antibody detects an ~140 kDa protein representing SAP97 from rat brain extract. Immunohistochemical staining of SAP97 in rat hippocampus with PA1-741 results in intense neuronal cell body and dendrite staining.

The PA1-741 immunizing peptide corresponds to amino acids 115-133 from rat SAP97. This sequence is 84% and 74% conserved in mouse and human SAP97, respectively.

PA1-741 can be used with blocking peptide PEP-055.

Target Information

This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats.

Nature communications

Kay Y,Tsan L,Davis EA,Tian C,Décarie-Spain L,Sadybekov A,Pushkin AN,Katritch V,Kanoski SE,Herring BE

PA1-741 was used in Western Blot to identify a cell-type-specific synaptic regulatory mechanism in the dentate gyrus that, when disrupted, impairs contextual information processing in rats.

Thu Feb 10 00:00:00 UTC 2022

Schizophrenia-associated SAP97 mutations increase glutamatergic synapse strength in the dentate gyrus and impair contextual episodic memory in rats.

Nature communications

Kay Y,Tsan L,Davis EA,Tian C,Décarie-Spain L,Sadybekov A,Pushkin AN,Katritch V,Kanoski SE,Herring BE

PA1-741 was used in Western Blot to identify a cell-type-specific synaptic regulatory mechanism in the dentate gyrus that, when disrupted, impairs contextual information processing in rats.

Thu Feb 10 00:00:00 UTC 2022

Quantitative fragmentomics allow affinity mapping of interactomes.

Nature communications

Gogl G,Zambo B,Kostmann C,Cousido-Siah A,Morlet B,Durbesson F,Negroni L,Eberling P,Jané P,Nominé Y,Zeke A,Østergaard S,Monsellier É,Vincentelli R,Travé G

PA1-741 was used in Western Blotting to show that interactome hijacking by the viral PBM of human papillomavirus E6 oncoprotein substantially impacts the host cell proteome beyond immediate E6 binders, illustrating the complex system-wide relationship between interactome and function.

Sat Sep 17 00:00:00 UTC 2022

Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.

American journal of physiology. Cell physiology

Christensen IB,Mogensen EN,Damkier HH,Praetorius J

PA1-741 was used in Immunohistochemistry to understand the polarity choroid plexus epithelial cells in the luminal plasma membrane domain.

Tue May 01 00:00:00 UTC 2018

Proteomic Analysis of Dendritic Filopodia-Rich Fraction Isolated by Telencephalin and Vitronectin Interaction.

Frontiers in synaptic neuroscience

Furutani Y,Yoshihara Y

PA1-741 was used in Immunocytochemistry-immunoflourescence to examine the molecular constituents of dendritic filopodia.

Wed Sep 30 00:00:00 UTC 2020

SAP97 regulates behavior and expression of schizophrenia risk enriched gene sets in mouse hippocampus.

PloS one

Gupta P,Uner OE,Nayak S,Grant GR,Kalb RG

Published figure using SAP97 polyclonal antibody (Product # PA1-741) in Western Blot

Tue Jan 08 00:00:00 UTC 2019

N-terminal SAP97 isoforms differentially regulate synaptic structure and postsynaptic surface pools of AMPA receptors.

Hippocampus

Goodman L,Baddeley D,Ambroziak W,Waites CL,Garner CC,Soeller C,Montgomery JM

PA1741 was used in immunocytochemistry to investigate diverging presynaptic and postsynaptic roles of SAP97 N-terminal isoforms in synapse maturation and plasticity

Thu Jun 01 00:00:00 UTC 2017

SAP97 Binding Partner CRIPT Promotes Dendrite Growth In Vitro and In Vivo.

eNeuro

Zhang L,Jablonski AM,Mojsilovic-Petrovic J,Ding H,Seeholzer S,Newton IP,Nathke I,Neve R,Zhai J,Shang Y,Zhang M,Kalb RG

Published figure using SAP97 polyclonal antibody (Product # PA1-741) in Immunoprecipitation

Wed Jul 25 00:00:00 UTC 2018

Upregulation of μ3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.

Cell reports

Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

Published figure using SAP97 polyclonal antibody (Product # PA1-741) in Immunocytochemistry

Tue Sep 06 00:00:00 UTC 2016

Dietary and donepezil modulation of mTOR signaling and neuroinflammation in the brain.

Biochimica et biophysica acta

Dasuri K,Zhang L,Kim SO,Bruce-Keller AJ,Keller JN

PA1-741 was used in western blot to analyze modulation by donepezil and diet on mTOR signaling and neuroinflammation in the brain by donepezil and diet

Mon Feb 01 00:00:00 UTC 2016

Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.

Molecular and cellular neurosciences

Zhang L,Hsu FC,Mojsilovic-Petrovic J,Jablonski AM,Zhai J,Coulter DA,Kalb RG

PA1-741 was used in western blot to demonstrate that the dendrite branching promoting action of full length SAP97 is dependent on the PDZ3 domain

Sun Mar 01 00:00:00 UTC 2015

Structure-function analysis of SAP97, a modular scaffolding protein that drives dendrite growth.

Molecular and cellular neurosciences

Zhang L,Hsu FC,Mojsilovic-Petrovic J,Jablonski AM,Zhai J,Coulter DA,Kalb RG

PA1-741 was used in western blot to demonstrate that the dendrite branching promoting action of full length SAP97 is dependent on the PDZ3 domain

Sun Mar 01 00:00:00 UTC 2015

Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif.

PloS one

Bender J,Engeholm M,Ederer MS,Breu J,Møller TC,Michalakis S,Rasko T,Wanker EE,Biel M,Martinez KL,Wurst W,Deussing JM

PA1-741 was used in immunocytochemistry to identify modulators of corticotropin-releasing hormone receptor type 1 signaling

Tue May 24 00:00:00 UTC 2016

Sequential delivery of synaptic GluA1- and GluA4-containing AMPA receptors (AMPARs) by SAP97 anchored protein complexes in classical conditioning.

The Journal of biological chemistry

Zheng Z,Keifer J

PA1-741 was used in immunohistochemistry to study the role of SAP97 in the sequential delivery of synaptic GluA1 and GluA4 AMPAR subunits in classical conditioning

Fri Apr 11 00:00:00 UTC 2014

Sequential delivery of synaptic GluA1- and GluA4-containing AMPA receptors (AMPARs) by SAP97 anchored protein complexes in classical conditioning.

The Journal of biological chemistry

Zheng Z,Keifer J

PA1-741 was used in immunohistochemistry to study the role of SAP97 in the sequential delivery of synaptic GluA1 and GluA4 AMPAR subunits in classical conditioning

Fri Apr 11 00:00:00 UTC 2014

Sequential delivery of synaptic GluA1- and GluA4-containing AMPA receptors (AMPARs) by SAP97 anchored protein complexes in classical conditioning.

The Journal of biological chemistry

Zheng Z,Keifer J

PA1-741 was used in immunohistochemistry to study the role of SAP97 in the sequential delivery of synaptic GluA1 and GluA4 AMPAR subunits in classical conditioning

Fri Apr 11 00:00:00 UTC 2014

Differential Changes in Postsynaptic Density Proteins in Postmortem Huntington's Disease and Parkinson's Disease Human Brains.

Journal of neurodegenerative diseases

Fourie C,Kim E,Waldvogel H,Wong JM,McGregor A,Faull RL,Montgomery JM

Published figure using SAP97 polyclonal antibody (Product # PA1-741) in Immunohistochemistry

Fri Aug 28 00:00:00 UTC 2015

Differential Changes in Postsynaptic Density Proteins in Postmortem Huntington's Disease and Parkinson's Disease Human Brains.

Journal of neurodegenerative diseases

Fourie C,Kim E,Waldvogel H,Wong JM,McGregor A,Faull RL,Montgomery JM

Published figure using SAP97 polyclonal antibody (Product # PA1-741) in Immunohistochemistry

Fri Aug 28 00:00:00 UTC 2015

Adenomatous polyposis coli regulates oligodendroglial development.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lang J,Maeda Y,Bannerman P,Xu J,Horiuchi M,Pleasure D,Guo F

PA1-741 was used in immunohistochemistry and western blot to study the mechanisms by which the the gut tumor suppressor APC regulates the development of oligodendroglia

Wed Feb 13 00:00:00 UTC 2013

Adenomatous polyposis coli regulates oligodendroglial development.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Lang J,Maeda Y,Bannerman P,Xu J,Horiuchi M,Pleasure D,Guo F

PA1-741 was used in immunohistochemistry and western blot to study the mechanisms by which the the gut tumor suppressor APC regulates the development of oligodendroglia

Wed Feb 13 00:00:00 UTC 2013

Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Murata Y,Constantine-Paton M

PA1-741 was used in western blot to examine the roles of SAP102 in cortical synapse development

Wed Mar 13 00:00:00 UTC 2013

Increased [³H]D-aspartate release and changes in glutamate receptor expression in the hippocampus of the mnd mouse.

Journal of neuroscience research

Bigini P,Milanese M,Gardoni F,Longhi A,Bonifacino T,Barbera S,Fumagalli E,Di Luca M,Mennini T,Bonanno G

PA1-741 was used in western blot to study the role of Cln8 gene in hippocampal excitabilit and the glutamatergic transmission system

Fri Jun 01 00:00:00 UTC 2012

Cognitive impairment in humanized APP×PS1 mice is linked to Aβ(1-42) and NOX activation.

Neurobiology of disease

Bruce-Keller AJ,Gupta S,Knight AG,Beckett TL,McMullen JM,Davis PR,Murphy MP,Van Eldik LJ,St Clair D,Keller JN

PA1-741 was used in western blot to investigate the relationship of cognitive impairment with NADPH oxidase activation and A beta deposition in a mouse Alzheimer disease model

Thu Dec 01 00:00:00 UTC 2011

Pals1 is a major regulator of the epithelial-like polarization and the extension of the myelin sheath in peripheral nerves.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ozçelik M,Cotter L,Jacob C,Pereira JA,Relvas JB,Suter U,Tricaud N

PA1-741 was used in immunohistochemistry to study the role of Pals1 in cellular polarization and the structure of myelin sheath in peripheral nerves

Wed Mar 17 00:00:00 UTC 2010

A specific requirement of Arc/Arg3.1 for visual experience-induced homeostatic synaptic plasticity in mouse primary visual cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Gao M,Sossa K,Song L,Errington L,Cummings L,Hwang H,Kuhl D,Worley P,Lee HK

PA1-741 was used in western blot to investigate the importance of Arc/Arg3.1 in visual experience-induced synaptic plasticity in mouse primary visual cortex

Wed May 26 00:00:00 UTC 2010

GluR1 controls dendrite growth through its binding partner, SAP97.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhou W,Zhang L,Guoxiang X,Mojsilovic-Petrovic J,Takamaya K,Sattler R,Huganir R,Kalb R

PA1-741 was used in immunocytochemistry and western blot to demonstrate that GluR1 controls dendrite growth by binding with SAP97

Wed Oct 08 00:00:00 UTC 2008

GluR1 controls dendrite growth through its binding partner, SAP97.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhou W,Zhang L,Guoxiang X,Mojsilovic-Petrovic J,Takamaya K,Sattler R,Huganir R,Kalb R

PA1-741 was used in immunocytochemistry and western blot to demonstrate that GluR1 controls dendrite growth by binding with SAP97

Wed Oct 08 00:00:00 UTC 2008

GluR1 controls dendrite growth through its binding partner, SAP97.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhou W,Zhang L,Guoxiang X,Mojsilovic-Petrovic J,Takamaya K,Sattler R,Huganir R,Kalb R

PA1-741 was used in immunocytochemistry and western blot to demonstrate that GluR1 controls dendrite growth by binding with SAP97

Wed Oct 08 00:00:00 UTC 2008

GluR1 controls dendrite growth through its binding partner, SAP97.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Zhou W,Zhang L,Guoxiang X,Mojsilovic-Petrovic J,Takamaya K,Sattler R,Huganir R,Kalb R

PA1-741 was used in immunocytochemistry and western blot to demonstrate that GluR1 controls dendrite growth by binding with SAP97

Wed Oct 08 00:00:00 UTC 2008

Differential neuronal and glial expression of GluR1 AMPA receptor subunit and the scaffolding proteins SAP97 and 4.1N during rat cerebellar development.

The Journal of comparative neurology

Douyard J,Shen L,Huganir RL,Rubio ME

PA1-741 was used in immunohistochemistry to study the expression of GluR1 AMPA receptor subunit and the scaffolding proteins SAP97 and 4.1N during rat cerebellar development

Tue May 01 00:00:00 UTC 2007

A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels.

The Journal of biological chemistry

Leonoudakis D,Conti LR,Radeke CM,McGuire LM,Vandenberg CA

PA1-741 was used in immunoprecipitation to demonstrate the CASK function in trafficking and plasma membrane localization of Kir2 channels.

Fri Apr 30 00:00:00 UTC 2004

A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels.

The Journal of biological chemistry

Leonoudakis D,Conti LR,Radeke CM,McGuire LM,Vandenberg CA

PA1-741 was used in immunoprecipitation to demonstrate the CASK function in trafficking and plasma membrane localization of Kir2 channels.

Fri Apr 30 00:00:00 UTC 2004

A multiprotein trafficking complex composed of SAP97, CASK, Veli, and Mint1 is associated with inward rectifier Kir2 potassium channels.

The Journal of biological chemistry

Leonoudakis D,Conti LR,Radeke CM,McGuire LM,Vandenberg CA

PA1-741 was used in immunoprecipitation to demonstrate the CASK function in trafficking and plasma membrane localization of Kir2 channels.

Fri Apr 30 00:00:00 UTC 2004

Differential recruitment of Kv1.4 and Kv4.2 to lipid rafts by PSD-95.

The Journal of biological chemistry

Wong W,Schlichter LC

PA1-741 was used in western blot to study the role of PSD-95 in Kv channel recuitment and activity.

Fri Jan 02 00:00:00 UTC 2004

Affinity purification of PSD-95-containing postsynaptic complexes.

Journal of neurochemistry

Vinade L,Chang M,Schlief ML,Petersen JD,Reese TS,Tao-Cheng JH,Dosemeci A

PA1-741 was used in western blot to develop an affinity-based strategy for the purification of Triton-derived PSD fraction

Mon Dec 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: discs large homolog 1; discs large homolog 1, scribble cell polarity complex component; discs, large homolog 1; discs, large homolog 1 (Drosophila) transcript variant 6-v1; discs, large homolog 1 (Drosophila) transcript variant 6-v2; Disks large homolog 1; DKFZp761P0818; DKFZp781B0426; Drosophila discs-large tumor suppressor homologue (synapse associated protein); E-dlg/SAP97; Embryo-dlg/synapse-associated protein 97; hDlg; presynaptic protein SAP97; SAP-97; Synapse-associated protein 97

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Gene Aliases: B130052P05Rik; dJ1061C18.1.1; DLG1; DLGH1; E-dlg/SAP97; hdlg; mKIAA4187; SAP-97; SAP97

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UniProt ID: (Human) Q12959, (Rat) Q62696, (Mouse) Q811D0

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Entrez Gene ID: (Human) 1739, (Rat) 25252, (Mouse) 13383

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Function(s)

guanylate kinase activity phosphoprotein phosphatase activity protein binding protein C-terminus binding cytoskeletal protein binding ligand-gated ion channel activity potassium channel regulator activity protein kinase binding phosphatase binding mitogen-activated protein kinase kinase binding protein complex scaffold ionotropic glutamate receptor binding ion channel binding L27 domain binding cadherin binding involved in cell-cell adhesion PDZ domain binding kinase binding

Process(es)

ureteric bud development branching involved in ureteric bud morphogenesis immunological synapse formation endothelial cell proliferation lens development in camera-type eye T cell cytokine production actin filament organization cell adhesion chemical synaptic transmission nervous system development protein localization positive regulation of cell proliferation single organismal cell-cell adhesion peristalsis positive regulation of actin filament polymerization cortical actin cytoskeleton organization membrane raft organization regulation of myelination activation of protein kinase activity regulation of protein localization T cell activation negative regulation of T cell proliferation regulation of membrane potential amyloid precursor protein metabolic process receptor clustering positive regulation of potassium ion transport establishment or maintenance of epithelial cell apical/basal polarity negative regulation of mitotic cell cycle nucleotide phosphorylation reproductive structure development embryonic skeletal system morphogenesis tissue morphogenesis smooth muscle tissue development negative regulation of epithelial cell proliferation negative regulation of protein kinase B signaling hard palate development bicellular tight junction assembly protein localization to plasma membrane positive regulation of establishment of protein localization to plasma membrane receptor localization to synapse positive regulation of multicellular organism growth positive regulation of developmental growth negative regulation of transcription from RNA polymerase II promoter protein dephosphorylation mitotic cell cycle checkpoint establishment or maintenance of cell polarity regulation of cell shape viral process astral microtubule organization ion transmembrane transport cellular protein complex localization cortical microtubule organization GMP metabolic process GDP metabolic process establishment of centrosome localization negative regulation of ERK1 and ERK2 cascade regulation of NIK/NF-kappaB signaling regulation of sodium ion transmembrane transport negative regulation of p38MAPK cascade

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SAP97 Polyclonal Antibody

SAP97 Antibody (PA1-741) in ICC/IF

Product Details

PA1-741

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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