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SAPAP1 Antibody (PA5-88953) in WB
Western blot analysis of SAPAP1 in extracts of various cell lines. Samples were incubated with SAPAP1 Polyclonal antibody (Product # PA5-88953) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-88953
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human DLGAP1 (NP_0012296901)
Conjugate
Form
Liquid
Concentration
2.58 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805249
Product Specific Information
Immunogen sequence: MKGLSGSRSH HHGVTCDSAC DSLSHHSDRK PYLLSPVEHH PADHPYYTQR NSFQAECVGP FSDPLASSTF PRRHYTSQQE LKDECALVPR TLATKANRIP ANLLDQFERQ LPLSRDGYHT LQYKRTAVEH RSDSPGRIRH LVHSVQKLFT KSHSLEGPSK GSVNGGKASP DEAQAARYGK RSKSKERRAE PKARPSTSPG WWSSDDNLDG DMCIYHAPSG VMTMGRCPDR SASQYFLEAY NTISEQAVKA; Positive Samples: U-87MG, Mouse brain; Cellular Location: Cell junction, Cell membrane, Peripheral membrane protein, postsynaptic cell membrane, postsynaptic density, synapse
Target Information
Part of the postsynaptic scaffold in neuronal cells. Target binds the guanylate kinase-like domain of the PSD-95 family of synaptic ion channel clustering proteins; may play a role in protein-protein interactions at the synaptic junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAP-1; DAP-1 beta; discs large homolog associated protein 1; discs, large (Drosophila) homolog-associated protein 1; Disks large-associated protein 1; guanylate kinase associated protein; Guanylate kinase-associated protein; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95 associated protein 1; SAP90/PSD-95-associated protein 1; SAPAP1
Gene Aliases: 4933422O14Rik; 9630002F18; AI845682; AI848168; BB075781; D17Bwg0511e; DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1; DLGAP1A; DLGAP1B; GKAP; GKAP/SAPAP; GKPA/SAPAP; Kiaa4162; mKIAA4162; SAPAP1
UniProt ID: (Human) O14490, (Mouse) Q9D415
Entrez Gene ID: (Human) 9229, (Mouse) 224997
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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