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SAPK4 Antibody (720302) in ICC/IF
For immunofluorescence analysis, MCF-7 cells were fixed and permeabilized for detection of endogenous p38 MAPK13 using Anti- p38 MAPK13 Rabbit Polyclonal Antibody (Product # 720302, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of MAPK13 (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Pane... View More
Product Details
720302
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to human p38 MAPK13 delta [1) aa316-333 2) aa113-126]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2662066
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Cat
Target Information
MAPK13 (p38 delta) is a serine/threonine kinase that transduces a variety of extracellular signals, including cytokine and environmental stress responses. MAPK13 also regulates the differentiation of keratinocytes. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases, or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of SAPK4 encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MAP kinase 13; MAP kinase p38 delta; Mitogen-activated protein kinase 13; Mitogen-activated protein kinase p38 delta; Stress-activated protein kinase 4
Gene Aliases: MAPK 13; MAPK-13; MAPK13; p38delta; PRKM13; SAPK4
UniProt ID: (Human) O15264
Entrez Gene ID: (Human) 5603
MAPK cascade
transcription, DNA-templated
regulation of transcription, DNA-templated
response to stress
response to osmotic stress
cell cycle
peptidyl-serine phosphorylation
positive regulation of interleukin-6 production
intracellular signal transduction
vascular endothelial growth factor receptor signaling pathway
positive regulation of inflammatory response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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