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SARS-CoV-2 NSP1 Antibody (PA5-119598) in ICC/IF
Immunocytochemistry analysis of SARS-CoV-2 NSP1 in 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with SARS-CoV-2 NSP1 polyclonal antibody (Product # PA5-119598) using a dilution of 1:1,000 (green). Blue: Fluoroshield with DAPI.
Product Details
PA5-119598
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of SARS-CoV-2 (COVID-19) nsp1 (Strain:Wuhan-Hu-1). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913171
Product Specific Information
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target Information
Inhibits host translation by interacting with the 40S ribosomal subunit (PubMed:32680882). Nsp1 C terminus binds to and obstructs ribosomal mRNA entry tunnel (PubMed:32680882). Thereby inhibits antiviral response triggered by innate immunity or interferons (PubMed:32680882). The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COVID-19 Nsp1; COVID19 Nsp1; SARS-CoV-2 Nonstructural Protein 1; SARS-COV-2 NSP1; SARS-CoV2 NSP1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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