Invitrogen

SARS-CoV-2 Nucleocapsid Chimeric Recombinant Human Monoclonal Antibody (1A6)

SARS-CoV-2 Nucleocapsid Chimeric Antibody in Western Blot (WB)

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SARS-CoV-2 Nucleocapsid Chimeric Antibody (MA5-35941) in WB

Western Blot analysis of SARS Coronavirus Nucleocapsid was performed by loading His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli. The membrane was blotted with SARS-CoV-2 Nucleocapsid Chimeric Recombinant Monoclonal Antibody (Product # MA5-35941). A Peroxidase Goat anti-Human IgG, Fcy Fragment Specific secondary antibody at a 1:20,000 dilution was used to detect the primary antibody. ... View More

SARS-CoV-2 Nucleocapsid Chimeric Antibody in ELISA (ELISA)

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Product Details

MA5-35941

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

ELISA (ELISA)

1:10,000-1:50,000

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Human, Virus

Host/Isotype

Human / IgG1

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1A6

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2866553

Product Specific Information

Recombinant anti-SARS-CoV-2 Nucleoprotein Mouse ScFv is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal.

Target Information

SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus. The SARS-CoV-2 N protein is an abundant RNA-binding protein critical for viral genome packaging, yet the molecular details that involve this process are poorly understood. N protein contains three dynamic disordered regions that house putative transiently-helical binding motifs. The two folded domains interact minimally such that full-length N protein is a flexible and multivalent RNA-binding protein. SARS-CoV-2 causes illnesses ranging from the common cold to severe diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Homologous Ad26.COV2.S vaccination results in reduced boosting of humoral responses in hybrid immunity, but elicits antibodies of similar magnitude regardless of prior infection.

PLoS pathogens

Moyo-Gwete T,Richardson SI,Keeton R,Hermanus T,Spencer H,Manamela NP,Ayres F,Makhado Z,Motlou T,Tincho MB,Benede N,Ngomti A,Baguma R,Chauke MV,Mennen M,Adriaanse M,Skelem S,Goga A,Garrett N,Bekker LG,Gray G,Ntusi NAB,Riou C,Burgers WA,Moore PL

MA5-35941 was used in ELISA to highlight that multiple antigen exposures, whether through infection and vaccination or vaccination alone, result in similar boosts after Ad26.COV2.S vaccination.

Wed Nov 01 00:00:00 UTC 2023

A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels.

Scientific reports

Alves J,Engel L,de Vasconcelos Cabral R,Rodrigues EL,de Jesus Ribeiro L,Higa LM,da Costa Ferreira Júnior O,Castiñeiras TMPP,de Carvalho Leitão I,Tanuri A,Goueli SA,Zegzouti H

MA5-35941 was used in Inhibition Assay, ELISA, Lateral Flow, Western Blot to describe a homogeneous bioluminescent immunoassay based on the interaction between Fc-tagged SARS-CoV-2 Spike RBD and human ACE2.

Thu Sep 16 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: COVID-19 nucleocapsid; COVID19 Nucleocapsid; COVID19 Spike nucleocapsid; SARS-CoV-2 N

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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