Invitrogen

SARS-CoV-2 Spike Protein (RBD) Recombinant Human Monoclonal Antibody (P05DHu), Alexa Fluor™ 488, eBioscience™

SARS-CoV-2 Spike Protein (RBD) Antibody in Flow Cytometry (Flow)

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SARS-CoV-2 Spike Protein (RBD) Antibody (53-6490-82) in Flow

HEK 293 cells were transfected with a vector encoding full length spike protein from SARS CoV2 (pink histogram) or left untransfected (blue histogram) and then stained with 1 µg of Anti-SARS CoV2 Spike Protein RBD Recombinant Human Monoclonal Antibody, Alexa Fluor 488 (Product # 53-6490-82). Viable cells were used for analysis as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).

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Product Details

53-6490-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

Product Specifications

Species Reactivity

Virus

Host/Isotype

Human / IgG1, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

P05DHu

Immunogen

This antibody was developed using computationally derived variants of SARS-CoV antibody structures and is shown to bind Spike Protein S1 subunit (RBD) of SARS CoV2

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

proprietary buffer/PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

AB_2884046

Product Specific Information

Description: This antibody recognizes the RBD domain of SARS CoV2 Spike protein.

Applications Tested: This P05DHu Alexa Fluor™ 488 conjugate has been tested by flow cytometric analysis of HEK 293 cells transfected with a vector encoding full length spike protein from SARS CoV2. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2; SARS-CoV2

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