Invitrogen

SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (B46F)

SARS/SARS-CoV-2 Nucleocapsid Antibody in Immunohistochemistry (IHC)

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SARS/SARS-CoV-2 Nucleocapsid Antibody (MA1-7404) in IHC

Immunohistochemistry was performed on lung tissues and placenta tissues from a SARS-CoV-2 patient. Heat-induced antigen retrieval was performed at pH 9.0 and tissues were probed with SARS/SARS-CoV-2 Coronavirus NP Monoclonal Antibody (B46F) (Product # MA1-7404) at a dilution of 1:100. Immunohistochemical stainings were performed with Leica Bond Auto-Stainer. The antibody shows significant staining in human ... View More

SARS/SARS-CoV-2 Nucleocapsid Antibody in Immunocytochemistry (ICC/IF)

SARS/SARS-CoV-2 Nucleocapsid Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

SARS/SARS-CoV-2 Nucleocapsid Antibody in Immunohistochemistry, In vitro Assay (IHC, IV)

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Product Details

MA1-7404

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:50

Immunohistochemistry (IHC)

1:100

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

ELISA (ELISA)

1:20-1:200

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

B46F

Immunogen

SARS nucleoprotein preparation.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1018422

Product Specific Information

MA1-7404 is reactive with the NP of SARS-CoV-2 (COVID-19 virus) by ELISA. Negative for human coronaviruses 229E & OC43. ELISA dilution range suggested 1:20- 1:200.

The MA1-7404 antibody reacts with SARS Coronavirus nucleoprotein in viral and infected tissue samples.

MA1-7404 has been successfully used in ELISA, Western blot and Immunofluorescence applications.

The MA1-7404 antibody was raised against a SARS virus nucleoprotein preparation.

Target Information

SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus. The SARS-CoV-2 N protein is an abundant RNA-binding protein critical for viral genome packaging, yet the molecular details that involve this process are poorly understood. N protein contains three dynamic disordered regions that house putative transiently-helical binding motifs. The two folded domains interact minimally such that full-length N protein is a flexible and multivalent RNA-binding protein. SARS-CoV-2 causes illnesses ranging from the common cold to severe diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dextran sulfate from Leuconostoc mesenteroides B512F exerts potent antiviral activity against SARS-CoV-2 in vitro and in vivo.

Frontiers in microbiology

Andreu S,von Kobbe C,Delgado P,Ripa I,Buzón MJ,Genescà M,Gironès N,Del Moral-Salmoral J,Ramírez GA,Zúñiga S,Enjuanes L,López-Guerrero JA,Bello-Morales R

MA1-7404 was used in Immunohistochemistry to suggest that Dextran sulfate (DS) polysaccharides may be considered as a potential candidate for antiviral therapy against SARS-CoV-2.

Mon May 22 00:00:00 UTC 2023

Full protection from SARS-CoV-2 brain infection and damage in susceptible transgenic mice conferred by MVA-CoV2-S vaccine candidate.

Nature neuroscience

Villadiego J,García-Arriaza J,Ramírez-Lorca R,García-Swinburn R,Cabello-Rivera D,Rosales-Nieves AE,Álvarez-Vergara MI,Cala-Fernández F,García-Roldán E,López-Ogáyar JL,Zamora C,Astorgano D,Albericio G,Pérez P,Muñoz-Cabello AM,Pascual A,Esteban M,López-Barneo J,Toledo-Aral JJ

MA1-7404 was used in Immunohistochemistry-immunofluorescence to report a spatiotemporal description of SARS-CoV-2 infection and replication through the brain, in the susceptible transgenic K18-hACE2 mouse model of severe coronavirus disease 2019 (COVID-19).

Wed Feb 01 00:00:00 UTC 2023

SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron.

iScience

Wu JL,Kuan II,Guo JY,Hsu WC,Tang WC,Chan HJ,Chen YJ,Chen BC,Wu HC,Liao JC

MA1-7404 was used in Immunocytochemistry to suggest that N protein may have an additional role in SARS-CoV-2 pathogenesis.

Fri Feb 17 00:00:00 UTC 2023

Eruptive pyogenic granulomas following coronavirus disease 2019.

Journal of the European Academy of Dermatology and Venereology : JEADV

Maronese CA,Moltrasio C,Marletta DA,Genovese G,Marzano AV

Published figure using SARS/SARS-CoV-2 Nucleocapsid monoclonal antibody (Product # MA1-7404) in Immunohistochemistry

Wed Jun 01 00:00:00 UTC 2022

Detection of SARS-CoV-2 infection in thyroid follicular cells from a COVID-19 autopsy series.

European thyroid journal

Macedo S,Pestana A,Santos L,Neves C,Guimarães S,Duarte-Neto A,Dolhnikoff M,Saldiva P,Alves G,Oliveira R,Cabanes D,Carneiro F,Sobrinho-Simões M,Soares P

MA1-7404 was used in Immunohistochemistry to indicate that thyrocytes can be directly infected by SARS-CoV-2 since we detected the presence of SARS-CoV-2 genome in follicular cells.

Mon Aug 01 00:00:00 UTC 2022

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in Chronic Obstructive Pulmonary Disease.

Microbiology spectrum

Osan J,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

MA1-7404 was used in Immunohistochemistry to determine whether SARS-CoV-2's cellular tropism plays a critical role in severe pathophysiology in the lung.

Wed Aug 31 00:00:00 UTC 2022

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in Chronic Obstructive Pulmonary Disease.

Microbiology spectrum

Osan J,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

MA1-7404 was used in Immunohistochemistry to determine whether SARS-CoV-2's cellular tropism plays a critical role in severe pathophysiology in the lung.

Wed Aug 31 00:00:00 UTC 2022

Evidence of SARS-CoV-2 spike protein on retrieved thrombi from COVID-19 patients.

Journal of hematology & oncology

De Michele M,d'Amati G,Leopizzi M,Iacobucci M,Berto I,Lorenzano S,Mazzuti L,Turriziani O,Schiavo OG,Toni D

MA1-7404 was used in Immunohistochemistry to support the hypothesis that free SP, besides the whole virus, may be the trigger of platelet activation and clot formation in COVID-19.

Tue Aug 16 00:00:00 UTC 2022

Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry.

Methods in molecular biology (Clifton, N.J.)

Lonardi S,Bugatti M,Valzelli A,Facchetti F

MA1-7404 was used in Immunohistochemistry to demonstrate the effectiveness of immunohistochemistry on analysing which cells are infected within tissue samples.

Tue May 17 00:00:00 UTC 2022

Clinical and in Vitro Evidence against Placenta Infection at Term by Severe Acute Respiratory Syndrome Coronavirus 2.

The American journal of pathology

Colson A,Depoix CL,Dessilly G,Baldin P,Danhaive O,Hubinont C,Sonveaux P,Debiève F

Published figure using SARS/SARS-CoV-2 Nucleocapsid monoclonal antibody (Product # MA1-7404) in Immunohistochemistry

Wed Sep 01 00:00:00 UTC 2021

TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection.

Microorganisms

Bortolotti D,Gentili V,Rizzo S,Schiuma G,Beltrami S,Strazzabosco G,Fernandez M,Caccuri F,Caruso A,Rizzo R

MA1-7404 was used in Immunocytochemistry to show that TLRs could be a potential target in controlling the infection in the early stages of the disease.

Thu Aug 26 00:00:00 UTC 2021

TLR3 and TLR7 RNA Sensor Activation during SARS-CoV-2 Infection.

Microorganisms

Bortolotti D,Gentili V,Rizzo S,Schiuma G,Beltrami S,Strazzabosco G,Fernandez M,Caccuri F,Caruso A,Rizzo R

MA1-7404 was used in Immunocytochemistry to show that TLRs could be a potential target in controlling the infection in the early stages of the disease.

Thu Aug 26 00:00:00 UTC 2021

Cardiac Fibrosis Is a Risk Factor for Severe COVID-19.

Frontiers in immunology

Mustroph J,Hupf J,Baier MJ,Evert K,Brochhausen C,Broeker K,Meindl C,Seither B,Jungbauer C,Evert M,Maier LS,Wagner S

Published figure using SARS/SARS-CoV-2 Nucleocapsid monoclonal antibody (Product # MA1-7404) in Immunocytochemistry

Thu Nov 25 00:00:00 UTC 2021

Cardiac Fibrosis Is a Risk Factor for Severe COVID-19.

Frontiers in immunology

Mustroph J,Hupf J,Baier MJ,Evert K,Brochhausen C,Broeker K,Meindl C,Seither B,Jungbauer C,Evert M,Maier LS,Wagner S

Published figure using SARS/SARS-CoV-2 Nucleocapsid monoclonal antibody (Product # MA1-7404) in Immunocytochemistry

Thu Nov 25 00:00:00 UTC 2021

Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.

Cell stem cell

Samuel RM,Majd H,Richter MN,Ghazizadeh Z,Zekavat SM,Navickas A,Ramirez JT,Asgharian H,Simoneau CR,Bonser LR,Koh KD,Garcia-Knight M,Tassetto M,Sunshine S,Farahvashi S,Kalantari A,Liu W,Andino R,Zhao H,Natarajan P,Erle DJ,Ott M,Goodarzi H,Fattahi F

MA1-7404 was used in Immunohistochemistry-wholemount to identify potentially novel cellular and molecular cascades triggered by RSV infection to exacerbate secondary pneumococcal pneumonia.

Thu Dec 03 00:00:00 UTC 2020

Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.

Cell stem cell

MA1-7404 was used in Immunohistochemistry-wholemount to identify potentially novel cellular and molecular cascades triggered by RSV infection to exacerbate secondary pneumococcal pneumonia.

Thu Dec 03 00:00:00 UTC 2020

Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents.

Laboratory investigation; a journal of technical methods and pathology

Best Rocha A,Stroberg E,Barton LM,Duval EJ,Mukhopadhyay S,Yarid N,Caza T,Wilson JD,Kenan DJ,Kuperman M,Sharma SG,Larsen CP

MA1-7404 was used in Immunohistochemistry to use immunohistochemical and in situ hybridization assays to detect the virus for diagnostic purposes and advancing understanding of the disease.

Sun Nov 01 00:00:00 UTC 2020

Perniosis during the COVID-19 pandemic: Negative anti-SARS-CoV-2 immunohistochemistry in six patients and comparison to perniosis before the emergence of SARS-CoV-2.

Journal of cutaneous pathology

Ko CJ,Harigopal M,Damsky W,Gehlhausen JR,Bosenberg M,Patrignelli R,McNiff JM

MA1-7404 was used in Immunohistochemistry to examine histopathologic findings in six consecutive cases from five patients received in mid-May to mid-June of 2020, evaluating immunohistochemical staining for the SARS-CoV-2 nucleocapsid protein.

Sun Nov 01 00:00:00 UTC 2020

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.

bioRxiv : the preprint server for biology

Osan JK,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

MA1-7404 was used in Immunohistochemistry to establish an airway epithelium model to study SARS-CoV-2 infection in healthy and COPD lung cells.

Thu Nov 12 00:00:00 UTC 2020

Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD.

bioRxiv : the preprint server for biology

Osan JK,Talukdar SN,Feldmann F,DeMontigny BA,Jerome K,Bailey KL,Feldmann H,Mehedi M

MA1-7404 was used in Immunohistochemistry to establish an airway epithelium model to study SARS-CoV-2 infection in healthy and COPD lung cells.

Thu Nov 12 00:00:00 UTC 2020

SARS-CoV-2 infection of human iPSC-derived cardiac cells predicts novel cytopathic features in hearts of COVID-19 patients.

bioRxiv : the preprint server for biology

Pérez-Bermejo JA,Kang S,Rockwood SJ,Simoneau CR,Joy DA,Ramadoss GN,Silva AC,Flanigan WR,Li H,Nakamura K,Whitman JD,Ott M,Conklin BR,McDevitt TC

MA1-7404 was used in Immunocytochemistry to investigate the affect of SARS-Cov-2 infection in patient-derived iPSC cardiac myocytes.

Sat Sep 12 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: COVID-19 nucleocapsid; COVID19 Nucleocapsid; COVID19 Spike nucleocapsid; SARS-CoV-2 N

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SARS/SARS-CoV-2 Nucleocapsid Monoclonal Antibody (B46F)

SARS/SARS-CoV-2 Nucleocapsid Antibody (MA1-7404) in IHC

Product Details

MA1-7404

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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