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FIGURE: 1 / 1
SBF1 Antibody (PA5-88490) in WB
Western blot analysis of SBF1 in extracts of various cell lines. Samples were incubated with SBF1 Polyclonal antibody (Product # PA5-88490) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88490
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1770 of human SBF1 (NP_0029632)
Conjugate
Form
Liquid
Concentration
1.73 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804949
Product Specific Information
Immunogen sequence: PPYDWELAQG PPEPPEEERS DGGAPQSRRR VVWPCYDSCP RAQPDAISRL LEELQRLETE LGQPAERWKD TWDRVKAAQR LEGRPDGRGT PSSLLVSTAP HHRRSLGVYL QEGPVGSTLS LSLDSDQSSG STTSGSRQAA R; Positive Samples: Raji, Jurkat, Rat brain; Cellular Location: Nucleus
Target Information
Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. According to PubMed:20937701, may function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DENN/MADD domain containing 7A; Inactive phosphatidylinositol 3-phosphatase 5; Myotubularin-related protein 5; Sbf1; SET-binding factor 1
Gene Aliases: CMT4B3; DENND7A; MTMR5; SBF1
UniProt ID: (Human) O95248
Entrez Gene ID: (Human) 6305, (Rat) 300147
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