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Invitrogen
SCD Monoclonal Antibody (CD.E10)
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FIGURE: 1 / 14
SCD Antibody (MA5-27542) in ICC/IF
Immunofluorescence of SCD was performed using HeLa WT and SCD KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cells were stained with... View More
Product Details
MA5-27542
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
CD.E10
Immunogen
Full length human recombinant protein against SCD1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2723611
Target Information
Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5., have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Acyl-CoA desaturase; Acyl-CoA desaturase 1; asebia; Delta(9)-desaturase; Delta(9)-desaturase 1; Delta-9 desaturase; Delta-9 desaturase 1; delta-9-desaturase; Fatty acid desaturase; Fatty acid desaturase 1; HGNC:10571; hSCD1; OTTHUMP00000020280; predicted protein of HQ0998; PRO0998; PRO1933; Stearoyl-CoA desaturase; stearoyl-CoA desaturase (delta-9-desaturase); Stearoyl-CoA desaturase 1; stearoyl-CoA desaturase opposite strand
Gene Aliases: AA589638; ab; AI265570; FADS5; hSCD1; MSTP008; SCD; Scd-1; SCD1; SCDOS
UniProt ID: (Human) O00767, (Mouse) P13516, (Rat) P07308
Entrez Gene ID: (Human) 6319, (Mouse) 20249, (Rat) 246074
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
positive regulation of cholesterol esterification
cholesterol esterification
negative regulation of growth of symbiont in host
defense response to Gram-positive bacterium
white fat cell differentiation
brown fat cell differentiation
oxidation-reduction process
monounsaturated fatty acid biosynthetic process
lipid biosynthetic process
unsaturated fatty acid biosynthetic process
long-chain fatty-acyl-CoA biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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