Invitrogen

SCF Polyclonal Antibody

2 Published Figures

4 References

View all (48) SCF antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Published Figures (2)

SCF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

SCF Antibody (PA5-20746) in IHC (P)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using SCF Polyclonal Antibody (Product # PA5-20746) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. ... View More

SCF Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

SCF Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-20746

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

2.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide from near the center of human SCF.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11151864

Product Specific Information

A suggested positive control is rat brain tissue lysate.

PA5-20746 can be used with blocking peptide PEP-0860.

Target Information

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Selective immunocapture reveals neoplastic human mast cells secrete distinct microvesicle- and exosome-like populations of KIT-containing extracellular vesicles.

Journal of extracellular vesicles

Pfeiffer A,Petersen JD,Falduto GH,Anderson DE,Zimmerberg J,Metcalfe DD,Olivera A

PA5-20746 was used in Western Blotting to show that selective KIT-immunocapture will enable the enrichment of specific huMC-derived EVs from complex human biosamples and facilitate an understanding of their in vivo functions and potential to serve as biomarkers of specific biological pathologies.

Thu Sep 01 00:00:00 UTC 2022

The pathogenic role of c-Kit+ mast cells in the spinal motor neuron-vascular niche in ALS.

Acta neuropathologica communications

Kovacs M,Alamón C,Maciel C,Varela V,Ibarburu S,Tarragó L,King PH,Si Y,Kwon Y,Hermine O,Barbeito L,Trias E

PA5-20746 was used in Immunohistochemistry to investigate the role of mast cells in amyotrophic lateral sclerosis.

Fri Aug 13 00:00:00 UTC 2021

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

PA5-20746 was used in Immunohistochemistry-immunofluorescence to analyse schwann cells phenotypes in sciatic nerves of amyotrophic lateral sclerosis patients and paralytic SOD1G93A rats to identify if similar and specific reactive schwann cells phenotypes exist.

Mon Jun 01 00:00:00 UTC 2020

Schwann cells orchestrate peripheral nerve inflammation through the expression of CSF1, IL-34, and SCF in amyotrophic lateral sclerosis.

Glia

Trias E,Kovacs M,King PH,Si Y,Kwon Y,Varela V,Ibarburu S,Moura IC,Hermine O,Beckman JS,Barbeito L

Mon Jun 01 00:00:00 UTC 2020

Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction.

International journal of molecular sciences

Dergilev KV,Shevchenko EK,Tsokolaeva ZI,Beloglazova IB,Zubkova ES,Boldyreva MA,Menshikov MY,Ratner EI,Penkov D,Parfyonova YV

PA5-20746 was used in Immunohistochemistry to generate a multilayered construct from adipose-derived mesenchymal stromal cells (MSCs) modified to secrete stem cell factor, SCF.

Wed Dec 16 00:00:00 UTC 2020

Cell Sheet Comprised of Mesenchymal Stromal Cells Overexpressing Stem Cell Factor Promotes Epicardium Activation and Heart Function Improvement in a Rat Model of Myocardium Infarction.

International journal of molecular sciences

Dergilev KV,Shevchenko EK,Tsokolaeva ZI,Beloglazova IB,Zubkova ES,Boldyreva MA,Menshikov MY,Ratner EI,Penkov D,Parfyonova YV

PA5-20746 was used in Immunohistochemistry to generate a multilayered construct from adipose-derived mesenchymal stromal cells (MSCs) modified to secrete stem cell factor, SCF.

Wed Dec 16 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: C-kit ligand; cloud gray; DKFZp686F2250; familial progressive hyperpigmentation 2; grizzle-belly; H-SCF; Hematopoietic growth factor KL; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; SCF; Steel factor; steel factor/kit ligand; Stem cell factor; stem cell factor KL-1

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Gene Aliases: blz; Clo; Con; contrasted; DCUA; DFNA69; FPH2; FPHH; Gb; Kitl; KITLG; KL-1; MGF; SCF; SF; SHEP7; Sl; SLF

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UniProt ID: (Human) P21583, (Mouse) P20826, (Rat) P21581

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Entrez Gene ID: (Human) 4254, (Mouse) 17311, (Rat) 60427

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity stem cell factor receptor binding protein binding growth factor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

Performance Guarantee

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SCF Polyclonal Antibody

SCF Antibody (PA5-20746) in IHC (P)

Product Details

PA5-20746

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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