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Invitrogen
SCN10A Monoclonal Antibody (S134), APC
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FIGURE: 1 / 3
SCN10A Antibody (MA5-45469) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with Nav1.8 monoclonal antibody (Product # MA5-45469) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav1.8 antibody (D) Composite.
Product Details
MA5-45469
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
S134
Immunogen
Fusion protein amino acids 1724-1956 of rat Nav1.8
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.15mM MES, pH 7.4, with 0.5M EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931923
Product Specific Information
1 µg/mL of MA5-45469 was sufficient for detection of Nav1.8 in 10 µg of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa. No cross reactivity against other Nav channels.
Target Information
Scn10a is a tetrodotoxin-resistant voltage-gated sodium channel alpha subunit. Epithelial sodium channels are amiloride-sensitive members of the Degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Scn10a mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Scn10a forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Scn10a also plays a role in neuropathic pain mechanisms. Structurally, members of Deg/ENaC superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddels syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1). Diseases associated with SCN10A include Episodic Pain Syndrome, Familial, 2 and Sodium Channelopathy-Related Small Fiber Neuropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mPN3; Peripheral nerve sodium channel 3; PN3; Sensory neuron sodium channel; Sodium channel protein type 10 subunit alpha; Sodium channel protein type X subunit alpha; sodium channel type X alpha polypeptide; sodium channel voltage-gated type X alpha polypeptide; sodium channel, voltage gated, type X alpha subunit; sodium channel, voltage-gated, type 10, alpha polypeptide; sodium channel, voltage-gated, type X, alpha polypeptide; sodium channel, voltage-gated, type X, alpha subunit; voltage gated sodium channel; Voltage-gated sodium channel subunit alpha Nav1.8
Gene Aliases: FEPS2; hPN3; Na(V)1.8; Nav1.8; PN3; SCN10A; SNS
UniProt ID: (Human) Q9Y5Y9, (Rat) Q62968, (Mouse) Q6QIY3
Entrez Gene ID: (Human) 6336, (Rat) 29571, (Mouse) 20264
regulation of heart rate
sodium ion transport
neuronal action potential
sensory perception of pain
regulation of ion transmembrane transport
sodium ion transmembrane transport
odontogenesis of dentin-containing tooth
regulation of cardiac muscle contraction
regulation of atrial cardiac muscle cell membrane depolarization
cardiac conduction
membrane depolarization during action potential
AV node cell to bundle of His cell communication
bundle of His cell to Purkinje myocyte communication
sensory perception
regulation of postsynaptic membrane potential
AV node cell action potential
bundle of His cell action potential
transport
ion transport
transmembrane transport
Performance Guarantee
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