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Invitrogen
SCRIB Polyclonal Antibody
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SCRIB Antibody (PA5-79959) in ICC/IF
Immunocytochemistry analysis of SCRIBBLE using anti-SCRIBBLE antibody (Product # PA5-79959) . SCRIBBLE was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-SCRIBBLE antibody (Product # PA5-79959) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained ... View More
Product Details
PA5-79959
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human SCRIBBLE recombinant protein (Position: F172-K409).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747074
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human COLO-320 whole cell, human 22RV1 whole cell, human SGC-7901 whole cell. ICC/IF: U20S cell. Flow: A549 cell, HepG2 cell.
Target Information
SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: circletail; hScrib; PDZ-domain protein scribble; Protein LAP4; Protein scribble homolog; S Vartul; Scribble; scribble homolog 1; scribbled homolog
Gene Aliases: AI118201; Crc; CRIB; CRIB1; KIAA0147; LAP4; mKIAA0147; RGD1565055; SCRB1; SCRIB; SCRIB1; VARTUL
UniProt ID: (Human) Q14160, (Mouse) Q80U72
Entrez Gene ID: (Human) 23513, (Mouse) 105782, (Rat) 362938
neural tube closure
positive regulation of receptor recycling
auditory receptor cell morphogenesis
multicellular organism development
asymmetric protein localization
cell proliferation
synaptic vesicle targeting
morphogenesis of embryonic epithelium
single organismal cell-cell adhesion
cell migration
cochlear nucleus development
cell differentiation
establishment of apical/basal cell polarity
wound healing
positive regulation of apoptotic process
astrocyte cell migration
negative regulation of mitotic cell cycle
synaptic vesicle endocytosis
positive chemotaxis
auditory receptor cell stereocilium organization
apoptotic process involved in morphogenesis
mammary gland duct morphogenesis
protein localization to adherens junction
activation of GTPase activity
viral process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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