Invitrogen

SDHB Monoclonal Antibody (21A11AE7)

SDHB Antibody in Immunocytochemistry (ICC/IF)

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SDHB Antibody (459230) in ICC/IF

Immunofluorescence analysis of SDHB was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SDHB Monoclonal Antibody (21A11AE7) (Product # 459230) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at... View More

SDHB Antibody in Immunohistochemistry (IHC)

Product Image Gallery

SDHB Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with SDHB siRNA and reduction in signal was observed in Western Blot using SDHB Monoclonal Antibody (21A11AE7) (Product # 459230). {KD}

Product Details

459230

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1 µg/ml

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg per 10^6 cells

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

21A11AE7

Immunogen

Bovine Heart Complex II, with traces of Complex III.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532233

Product Specific Information

Positive control: Human heart mitochondria.

Target Information

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.

Nature communications

Schmidt S,Stautner C,Vu DT,Heinz A,Regensburger M,Karayel O,Trümbach D,Artati A,Kaltenhäuser S,Nassef MZ,Hembach S,Steinert L,Winner B,Jürgen W,Jastroch M,Luecken MD,Theis FJ,Westmeyer GG,Adamski J,Mann M,Hiller K,Giesert F,Vogt Weisenhorn DM,Wurst W

459230 was used in Western Blot to demonstrate a correlation between OGDHC activity in our cellular model and the disease progression.

Thu Nov 23 00:00:00 UTC 2023

A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease.

Nature communications

459230 was used in Western Blot to demonstrate a correlation between OGDHC activity in our cellular model and the disease progression.

Thu Nov 23 00:00:00 UTC 2023

Cytoplasmic HIF-2α as tissue biomarker to identify metastatic sympathetic paraganglioma.

Scientific reports

Karakaya S,Gunnesson L,Elias E,Martos-Salvo P,Robledo M,Nilsson O,Wängberg B,Abel F,Påhlman S,Muth A,Mohlin S

459230 was used in Immunohistochemistryto propose that higher cytoplasmic HIF-2α expression could serve as a useful clinical marker to differentiate paragangliomas from pheochromocytomas, and may help predict outcome in sympathetic paraganglioma patients.

Tue Jul 18 00:00:00 UTC 2023

Chronic Hyperglycaemia Inhibits Tricarboxylic Acid Cycle in Rat Cardiomyoblasts Overexpressing Glucose Transporter Type 4.

International journal of molecular sciences

Stratmann B,Eggers B,Mattern Y,Silva de Carvalho T,Marcus K,Tschoepe D

459230 was used in Western Blot to study effects of a high glucose supply.

Wed Jun 29 00:00:00 UTC 2022

High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol.

Cell death & disease

Deitersen J,Berning L,Stuhldreier F,Ceccacci S,Schlütermann D,Friedrich A,Wu W,Sun Y,Böhler P,Berleth N,Mendiburo MJ,Seggewiß S,Anand R,Reichert AS,Monti MC,Proksch P,Stork B

459230 was used in Western Blot to search for autophagy-modulating compounds for possible cancer therapy using flow cytometry-based high-throughput screening.

Mon May 31 00:00:00 UTC 2021

Herba Houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade.

Nutrients

Cao K,Lv W,Liu X,Fan Y,Wang K,Feng Z,Liu J,Zang W,Xing L,Liu J

459230 was used in Western Blot to link the AMPK/PGC-1α/Nrf2 cascade to hyperlipidemia-induced liver and heart impairments and demonstrate the protective effect of HAE as an AMPK activator in the prevention of hyperlipidemia-related diseases.

Tue Jan 07 00:00:00 UTC 2020

Herba Houttuyniae Extract Benefits Hyperlipidemic Mice via Activation of the AMPK/PGC-1α/Nrf2 Cascade.

Nutrients

Cao K,Lv W,Liu X,Fan Y,Wang K,Feng Z,Liu J,Zang W,Xing L,Liu J

Tue Jan 07 00:00:00 UTC 2020

Metabolic Reprogramming in Astrocytes Distinguishes Region-Specific Neuronal Susceptibility in Huntington Mice.

Cell metabolism

Polyzos AA,Lee DY,Datta R,Hauser M,Budworth H,Holt A,Mihalik S,Goldschmidt P,Frankel K,Trego K,Bennett MJ,Vockley J,Xu K,Gratton E,McMurray CT

459230 was used in Western Blot to show that region-specific neuronal vulnerability is a substrate-driven response in astrocytes.

Tue Jun 04 00:00:00 UTC 2019

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Nucleic acids research

Van Haute L,Hendrick AG,D'Souza AR,Powell CA,Rebelo-Guiomar P,Harbour ME,Ding S,Fearnley IM,Andrews B,Minczuk M

Published figure using SDHB monoclonal antibody (Product # 459230) in Western Blot

Mon Nov 04 00:00:00 UTC 2019

L-Arabinose Elicits Gut-Derived Hydrogen Production and Ameliorates Metabolic Syndrome in C57BL/6J Mice on High-Fat-Diet.

Nutrients

Zhao L,Wang Y,Zhang G,Zhang T,Lou J,Liu J

459230 was used in Western Blot to show oral L-arabinose elicited immediate and robust release of hydrogen in mice in a dose-and-time-dependent manner while alleviating high-fat-diet induced metabolic syndrome.

Fri Dec 13 00:00:00 UTC 2019

The mito::mKate2 mouse: A far-red fluorescent reporter mouse line for tracking mitochondrial dynamics in vivo.

Genesis (New York, N.Y. : 2000)

Barrasso AP,Tong X,Poché RA

459230 was used in Immunohistochemistry-immunofluorescence to demonstrate the potential utility of mito::mKate2 for genetic mosaic analysis of mitochondrial phenotypes.

Thu Feb 01 00:00:00 UTC 2018

PIMT/NCOA6IP Deletion in the Mouse Heart Causes Delayed Cardiomyopathy Attributable to Perturbation in Energy Metabolism.

International journal of molecular sciences

Jia Y,Liu N,Viswakarma N,Sun R,Schipma MJ,Shang M,Thorp EB,Kanwar YS,Thimmapaya B,Reddy JK

459230 was used in Western Blotting to suggest that PIMT functions in cardiac energy metabolism by interacting with nuclear receptor coactivators and this property could be useful in the management of heart failure.

Wed May 16 00:00:00 UTC 2018

Mitochondrial Maturation in Human Pluripotent Stem Cell Derived Cardiomyocytes.

Stem cells international

Dai DF,Danoviz ME,Wiczer B,Laflamme MA,Tian R

459230 was used in immunocytochemistry to discover the development of mitochondria during prolonged in vitro culturing of human pluripotent stem cells derived cardiomyocytes

Thu Oct 01 00:00:00 UTC 2020

Activation of sirtuin 1 as therapy for the peroxisomal disease adrenoleukodystrophy.

Cell death and differentiation

Morató L,Ruiz M,Boada J,Calingasan NY,Galino J,Guilera C,Jové M,Naudí A,Ferrer I,Pamplona R,Serrano M,Portero-Otín M,Beal MF,Fourcade S,Pujol A

Published figure using SDHB monoclonal antibody (Product # 459230) in Western Blot

Sun Nov 01 00:00:00 UTC 2015

The transcriptional coregulator PGC-1β controls mitochondrial function and anti-oxidant defence in skeletal muscles.

Nature communications

Gali Ramamoorthy T,Laverny G,Schlagowski AI,Zoll J,Messaddeq N,Bornert JM,Panza S,Ferry A,Geny B,Metzger D

459230 was used in western blot to find the function of transcriptional coregulator PGC-1beta in mitochondria and anti-oxidant defense in skeletal muscles

Thu Dec 17 00:00:00 UTC 2015

D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.

Nature communications

Lin AP,Abbas S,Kim SW,Ortega M,Bouamar H,Escobedo Y,Varadarajan P,Qin Y,Sudderth J,Schulz E,Deutsch A,Mohan S,Ulz P,Neumeister P,Rakheja D,Gao X,Hinck A,Weintraub ST,DeBerardinis RJ,Sill H,Dahia PL,Aguiar RC

Published figure using SDHB monoclonal antibody (Product # 459230) in Western Blot

Thu Jul 16 00:00:00 UTC 2015

Prevention and reversal of severe mitochondrial cardiomyopathy by gene therapy in a mouse model of Friedreich's ataxia.

Nature medicine

Perdomini M,Belbellaa B,Monassier L,Reutenauer L,Messaddeq N,Cartier N,Crystal RG,Aubourg P,Puccio H

459230 was used in western blot to assess the use of gene therapy in treating Friedreich's ataxia cardiomyopathy in a mouse model.

Thu May 01 00:00:00 UTC 2014

Mitochondria and tumor progression in ulcerative colitis.

Journal of the National Cancer Institute

Ussakli CH,Ebaee A,Binkley J,Brentnall TA,Emond MJ,Rabinovitch PS,Risques RA

Published figure using SDHB monoclonal antibody (Product # 459230) in Immunohistochemistry

Wed Aug 21 00:00:00 UTC 2013

Independent roles of methionine sulfoxide reductase A in mitochondrial ATP synthesis and as antioxidant in retinal pigment epithelial cells.

Free radical biology & medicine

Dun Y,Vargas J,Brot N,Finnemann SC

459230 was used in western blot to test if and how MsrA affects retinal pigment epithelium functionality.

Sun Dec 01 00:00:00 UTC 2013

β-Adrenergic stimulation does not activate p38 MAP kinase or induce PGC-1α in skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Kim SH,Asaka M,Higashida K,Takahashi Y,Holloszy JO,Han DH

459230 was used in western blot to test if the increases in PGC-α and mitochondrial biogenesis induced by endurance exercise are mediated by catecholamines.

Mon Apr 15 00:00:00 UTC 2013

High-throughput screening for growth inhibitors using a yeast model of familial paraganglioma.

PloS one

Bancos I,Bida JP,Tian D,Bundrick M,John K,Holte MN,Her YF,Evans D,Saenz DT,Poeschla EM,Hook D,Georg G,Maher LJ

459230 was used in western blot to identify compounds that are toxic to succinate dehydrogenase mutant cells using a yeast model of paraganglioma.

Tue Sep 03 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: FLJ92337; IP; iron-sulfur subunit; Iron-sulfur subunit of complex II; Malate dehydrogenase [quinone] iron-sulfur subunit; OTTHUMP00000044624; succinate dehydrogenase [ubiquinone] iron-sulfur subunit; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex subunit B; succinate dehydrogenase complex subunit B, iron sulfur (Ip); succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

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Gene Aliases: 0710008N11Rik; BOS_2308; CWS2; IP; PGL4; SDH; SDH1; SDH2; SDHB; SDHIP

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UniProt ID: (Human) P21912, (Bovine) Q3T189, (Mouse) Q9CQA3, (Rat) P21913

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Entrez Gene ID: (Human) 6390, (Bovine) 286840, (Mouse) 67680, (Rat) 298596

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Function(s)

protein binding succinate dehydrogenase (ubiquinone) activity electron carrier activity metal ion binding ubiquinone binding 2 iron, 2 sulfur cluster binding 3 iron, 4 sulfur cluster binding 4 iron, 4 sulfur cluster binding oxidoreductase activity iron-sulfur cluster binding

Process(es)

tricarboxylic acid cycle succinate metabolic process aerobic respiration respiratory electron transport chain electron transport chain transport oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SDHB Monoclonal Antibody (21A11AE7)

SDHB Antibody (459230) in ICC/IF

Product Details

459230

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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