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Product Details
29417-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: EVGKPEDEAS GSFFKQIDSS PVGGETDETT VSQNYRGSVS QPSTPSPPKP TGIFQTSANS SFEPVKSHLV GVKPFEADRA NVVGEVRETC VRQKQCRPAA ALPDASPGNL EQPPDNMETL CAPQVCPLPL NSTTEAVHML PHAGAPPLDT VYPAPEKRPS ARTQGPVKCE SPA
Target Information
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p250; protein SEC16 homolog A; Protein transport protein Sec16A; SEC16 homolog A
Gene Aliases: KIAA0310; p250; SEC16; SEC16A; SEC16L
UniProt ID: (Human) O15027
Entrez Gene ID: (Human) 9919
Performance Guarantee
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