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SEC23A Antibody (PA5-143936) in ICC/IF
Immunocytochemistry analysis of SEC23A in HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in SEC23A Polyclonal antibody (Product # PA5-143936) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriat... View More
Product Details
PA5-143936
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human SEC23A recombinant protein (Position: R190-Q579).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3075150
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human HEK293 whole cell, human A549 whole cell, human PC-3 whole cell, rat liver tissue, rat stomach tissue, rat lung tissue, mouse liver tissue. IHC: human placenta tissue. ICC/IF: HepG2 cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family. It is part of a protein complex and found in the ribosome-free transitional face of the endoplasmic reticulum (ER) and associated vesicles. This protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The encoded protein is suggested to play a role in the ER-Golgi protein trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hSec23A; MGC26267; Protein transport protein Sec23A; Sec23 homolog A, COPII coat complex component; SEC23-related protein A; SEC23A
Gene Aliases: CLSD; Msec23; Sec23; SEC23A; Sec23r
UniProt ID: (Human) Q15436, (Mouse) Q01405
Entrez Gene ID: (Human) 10484, (Mouse) 20334, (Rat) 58817
transport
intracellular protein transport
ER to Golgi vesicle-mediated transport
protein transport
vesicle-mediated transport
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class II
COPII vesicle coating
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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