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Invitrogen
SEC61A1 Polyclonal Antibody
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FIGURE: 1 / 3
SEC61A1 Antibody (PA5-115879) in ICC/IF
Immunocytochemistry analysis of SEC61A1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115879) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115879
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SEC61A1(Accession P61619), corresponding to amino acid residues S443-F476.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2900513
Product Specific Information
Antibody detects endogenous levels of total SEC61A.
Target Information
The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: protein transport protein SEC61 alpha subunit; protein transport protein Sec61 subunit alpha; Protein transport protein Sec61 subunit alpha isoform 1; Sec61 alpha 1 subunit; Sec61 alpha subunit homolog; Sec61 alpha-1; sec61 homolog; SEC61, alpha subunit; transport protein sec61 alpha subunit
Gene Aliases: AA408394; AA410007; HSEC61; SEC61; SEC61A; SEC61A1
UniProt ID: (Human) P61619, (Mouse) P61620, (Rat) P61621
Entrez Gene ID: (Human) 29927, (Mouse) 53421, (Rat) 80843
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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