Proteintech

SEC61B Polyclonal Antibody

SEC61B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

SEC61B Antibody (15087-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SEC61B antibody (Product # 15087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

SEC61B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SEC61B Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15087-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SEC61B Fusion Protein Ag7169 (1-96 aa encoded by BC001734)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MPGPTPSGT NVGSSGRSPS KAVAARAAGS TVRQRKNASC GTRSAGRTTS AGTGGMWRFY TEDSPGLKVG PVPVLVMSLL FIASVFMLHI WGKYTRS (1-96 aa encoded by B C001734)

Target Information

The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Vps9d1 regulates tubular endosome formation through specific activation of Rab22A.

Journal of cell science

Nakashima S,Matsui T,Fukuda M

Wed Mar 15 00:00:00 UTC 2023

A regulatory circuit comprising the CBP and SIRT7 regulates FAM134B-mediated ER-phagy.

The Journal of cell biology

Wang X,Jiang X,Li B,Zheng J,Guo J,Gao L,Du M,Weng X,Li L,Chen S,Zhang J,Fang L,Liu T,Wang L,Liu W,Neculai D,Sun Q

Mon May 01 00:00:00 UTC 2023

Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of Aβ in neurons, yielding senile plaques.

Nature neuroscience

Lee JH,Yang DS,Goulbourne CN,Im E,Stavrides P,Pensalfini A,Chan H,Bouchet-Marquis C,Bleiwas C,Berg MJ,Huo C,Peddy J,Pawlik M,Levy E,Rao M,Staufenbiel M,Nixon RA

Wed Jun 01 00:00:00 UTC 2022

Transmembrane Prolyl 4-Hydroxylase is a Novel Regulator of Calcium Signaling in Astrocytes.

eNeuro

Byts N,Sharma S,Laurila J,Paudel P,Miinalainen I,Ronkainen VP,Hinttala R,Törnquist K,Koivunen P,Myllyharju J

Fri Jun 18 00:00:00 UTC 2021

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease.

Acta neuropathologica communications

Rimal S,Li Y,Vartak R,Geng J,Tantray I,Li S,Huh S,Vogel H,Glabe C,Grinberg LT,Spina S,Seeley WW,Guo S,Lu B

Mon Oct 18 00:00:00 UTC 2021

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease.

Acta neuropathologica communications

Rimal S,Li Y,Vartak R,Geng J,Tantray I,Li S,Huh S,Vogel H,Glabe C,Grinberg LT,Spina S,Seeley WW,Guo S,Lu B

Mon Oct 18 00:00:00 UTC 2021

cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro.

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology

Yamada Y,Miyamoto T,Higuchi S,Ono M,Kobara H,Asaka R,Ando H,Suzuki A,Shiozawa T

Fri Jan 01 00:00:00 UTC 2021

QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis.

Science (New York, N.Y.)

You K,Wang L,Chou CH,Liu K,Nakata T,Jaiswal A,Yao J,Lefkovith A,Omar A,Perrigoue JG,Towne JE,Regev A,Graham DB,Xavier RJ

Fri Jan 01 00:00:00 UTC 2021

Propofol induces the elevation of intracellular calcium via morphological changes in intracellular organelles, including the endoplasmic reticulum and mitochondria.

European journal of pharmacology

Urabe T,Yanase Y,Motoike S,Harada K,Hide I,Tanaka S,Tsutsumi YM,Kawamoto M,Sakai N

Mon Oct 05 00:00:00 UTC 2020

Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.

Cell reports

Pensalfini A,Kim S,Subbanna S,Bleiwas C,Goulbourne CN,Stavrides PH,Jiang Y,Lee JH,Darji S,Pawlik M,Huo C,Peddy J,Berg MJ,Smiley JF,Basavarajappa BS,Nixon RA

Tue Nov 24 00:00:00 UTC 2020

Identification of a localized nonsense-mediated decay pathway at the endoplasmic reticulum.

Genes & development

Longman D,Jackson-Jones KA,Maslon MM,Murphy LC,Young RS,Stoddart JJ,Hug N,Taylor MS,Papadopoulos DK,Cáceres JF

Sat Aug 01 00:00:00 UTC 2020

CReP mediates selective translation initiation at the endoplasmic reticulum.

Science advances

Kastan JP,Dobrikova EY,Bryant JD,Gromeier M

Mon Jun 01 00:00:00 UTC 2020

Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.

Molecular therapy : the journal of the American Society of Gene Therapy

Wang X,Liu Z,Li G,Dang L,Huang S,He L,Ma Y,Li C,Liu M,Yang G,Huang X,Zhou F,Ma X

Wed Feb 05 00:00:00 UTC 2020

Intrinsically Disordered Protein TEX264 Mediates ER-phagy.

Molecular cell

Chino H,Hatta T,Natsume T,Mizushima N

Thu Jun 06 00:00:00 UTC 2019

A critical role of VMP1 in lipoprotein secretion.

eLife

Morishita H,Zhao YG,Tamura N,Nishimura T,Kanda Y,Sakamaki Y,Okazaki M,Li D,Mizushima N

Tue Sep 17 00:00:00 UTC 2019

Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.

The Journal of cell biology

Matsui T,Jiang P,Nakano S,Sakamaki Y,Yamamoto H,Mizushima N

Mon Aug 06 00:00:00 UTC 2018

The ATPase activity of Asna1/TRC40 is required for pancreatic progenitor cell survival.

Development (Cambridge, England)

Norlin S,Parekh V,Edlund H

Wed Jan 03 00:00:00 UTC 2018

The ATPase activity of Asna1/TRC40 is required for pancreatic progenitor cell survival.

Development (Cambridge, England)

Norlin S,Parekh V,Edlund H

Wed Jan 03 00:00:00 UTC 2018

Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine α-Amidating Monooxygenase.

Endocrinology

Mains RE,Blaby-Haas C,Rheaume BA,Eipper BA

Sun Jul 01 00:00:00 UTC 2018

Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport.

Diabetes

Norlin S,Parekh VS,Naredi P,Edlund H

Fri Jan 01 00:00:00 UTC 2016

Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport.

Diabetes

Norlin S,Parekh VS,Naredi P,Edlund H

Fri Jan 01 00:00:00 UTC 2016

Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport.

Diabetes

Norlin S,Parekh VS,Naredi P,Edlund H

Fri Jan 01 00:00:00 UTC 2016

Asna1/TRC40 Controls β-Cell Function and Endoplasmic Reticulum Homeostasis by Ensuring Retrograde Transport.

Diabetes

Norlin S,Parekh VS,Naredi P,Edlund H

Fri Jan 01 00:00:00 UTC 2016

Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.

The Journal of cell biology

Ninagawa S,Okada T,Sumitomo Y,Horimoto S,Sugimoto T,Ishikawa T,Takeda S,Yamamoto T,Suzuki T,Kamiya Y,Kato K,Mori K

Mon Nov 23 00:00:00 UTC 2015

Forcible destruction of severely misfolded mammalian glycoproteins by the non-glycoprotein ERAD pathway.

The Journal of cell biology

Ninagawa S,Okada T,Sumitomo Y,Horimoto S,Sugimoto T,Ishikawa T,Takeda S,Yamamoto T,Suzuki T,Kamiya Y,Kato K,Mori K

Mon Nov 23 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: protein translocation complex beta; protein transport protein SEC61 beta subunit; Protein transport protein Sec61 subunit beta; sec61 beta; Sec61 beta subunit; Sec61 complex, beta subunit

View more View less

Gene Aliases: 1190006C12Rik; AI326121; AW122942; SEC61B

View more View less

UniProt ID: (Human) P60468, (Mouse) Q9CQS8

View more View less

Entrez Gene ID: (Human) 10952, (Mouse) 66212, (Rat) 298068

View more View less

Function(s)

protein binding poly(A) RNA binding epidermal growth factor binding ribosome binding molecular_function

Process(es)

protein import into nucleus, translocation transport intracellular protein transport protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol biological_process IRE1-mediated unfolded protein response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

SEC61B Polyclonal Antibody

SEC61B Antibody (15087-1-AP) in ICC/IF

Product Details

15087-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!