Invitrogen

SEC61B Polyclonal Antibody

4 Published Figures

8 References

View all (6) SEC61B antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

SEC61B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

SEC61B Antibody (PA3-015) in ICC/IF

Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (Product # PA3-015) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

SEC61B Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Product Details

PA3-015

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250

View 3 publications 3 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Pig, Rat

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2239072

Product Specific Information

PA3-015 detects human, canine and porcine, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.

PA3-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~14 kDa protein representing sec61 beta from pig microsomes.

The PA3-015 immunogen is a synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta. This sequence is completely conserved between human, mouse, and canine.

Target Information

The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Juvenile CLN3 disease is a lysosomal cholesterol storage disorder: similarities with Niemann-Pick type C disease.

EBioMedicine

Chen J,Soni RK,Xu Y,Simoes S,Liang FX,DeFreitas L,Hwang R,Montesinos J,Lee JH,Area-Gomez E,Nandakumar R,Vardarajan B,Marquer C

PA3-015 was used in Western Blotting to support that JNCL is a lysosomal cholesterol storage disorder.

Thu Jun 01 00:00:00 UTC 2023

Genomic Action of Sigma-1 Receptor Chaperone Relates to Neuropathic Pain.

Molecular neurobiology

Wang SM,Goguadze N,Kimura Y,Yasui Y,Pan B,Wang TY,Nakamura Y,Lin YT,Hogan QH,Wilson KL,Su TP,Wu HE

Published figure using SEC61B polyclonal antibody (Product # PA3-015) in Immunocytochemistry

Tue Jun 01 00:00:00 UTC 2021

The Alzheimer's disease-associated C99 fragment of APP regulates cellular cholesterol trafficking.

The EMBO journal

Montesinos J,Pera M,Larrea D,Guardia-Laguarta C,Agrawal RR,Velasco KR,Yun TD,Stavrovskaya IG,Xu Y,Koo SY,Snead AM,Sproul AA,Area-Gomez E

PA3-015 was used in Immunocytochemistry to investigate the link between cholesterol homeostasis and cleavage of the amyloid precursor protein (APP), and how this relationship relates to Alzheimer's disease (AD) pathogenesis.

Thu Oct 15 00:00:00 UTC 2020

The Alzheimer's disease-associated C99 fragment of APP regulates cellular cholesterol trafficking.

The EMBO journal

Montesinos J,Pera M,Larrea D,Guardia-Laguarta C,Agrawal RR,Velasco KR,Yun TD,Stavrovskaya IG,Xu Y,Koo SY,Snead AM,Sproul AA,Area-Gomez E

Thu Oct 15 00:00:00 UTC 2020

mTORC1 phosphorylates LARP6 to stimulate type I collagen expression.

Scientific reports

Zhang Y,Stefanovic B

PA3015 was used in western blot to find that mTORC1 phosphorylates La ribonucleoprotein domain family, member 6 on S348 and S409

Mon Jan 23 00:00:00 UTC 2017

mTORC1 phosphorylates LARP6 to stimulate type I collagen expression.

Scientific reports

Zhang Y,Stefanovic B

PA3015 was used in western blot to find that mTORC1 phosphorylates La ribonucleoprotein domain family, member 6 on S348 and S409

Mon Jan 23 00:00:00 UTC 2017

Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins.

Development & reproduction

Yong H,Oh HI,Lee SH,Cheong HT,Yang BK,Park CK

PA3-015 was used in Immunocytochemistry-immunoflourescence to demonstrate that epidermal growth factor plays an important role in the systems for oocyte maturation with endoplasmic reticulum and Golgi apparatus.

Thu Jun 01 00:00:00 UTC 2017

Characterization of binding of LARP6 to the 5' stem-loop of collagen mRNAs: implications for synthesis of type I collagen.

RNA biology

Stefanovic L,Longo L,Zhang Y,Stefanovic B

PA3-015 was used in immunoprecipitation and western blot to investigate the contribution of the unique sequence of LARP6 to type I collagen biosynthesis

Mon Oct 26 00:00:00 UTC 2015

Characterization of binding of LARP6 to the 5' stem-loop of collagen mRNAs: implications for synthesis of type I collagen.

RNA biology

Stefanovic L,Longo L,Zhang Y,Stefanovic B

PA3-015 was used in immunoprecipitation and western blot to investigate the contribution of the unique sequence of LARP6 to type I collagen biosynthesis

Mon Oct 26 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: protein translocation complex beta; protein translocation complex beta subunit; protein transport protein SEC61 beta subunit; Protein transport protein Sec61 subunit beta; sec61 beta; Sec61 beta subunit; Sec61 complex, beta subunit

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Gene Aliases: 1190006C12Rik; AI326121; AW122942; SEC61B

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UniProt ID: (Human) P60468, (Dog) P60467, (Mouse) Q9CQS8

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Entrez Gene ID: (Human) 10952, (Dog) 404018, (Pig) 100511362, (Mouse) 66212, (Rat) 298068

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Function(s)

protein binding poly(A) RNA binding epidermal growth factor binding P-P-bond-hydrolysis-driven protein transmembrane transporter activity ribosome binding molecular_function

Process(es)

protein import into nucleus, translocation transport intracellular protein transport protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol biological_process IRE1-mediated unfolded protein response protein transmembrane transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SEC61B Polyclonal Antibody

SEC61B Antibody (PA3-015) in ICC/IF

Product Details

PA3-015

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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