Proteintech

SEC63 Polyclonal Antibody

SEC63 Antibody in Immunocytochemistry (ICC/IF)

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SEC63 Antibody (13978-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (Product # 13978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SEC63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SEC63 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

SEC63 Antibody in Immunoprecipitation (IP)

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Product Details

13978-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SEC63 Fusion Protein Ag5021 (409-760 aa encoded by BC047221)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: IQDLVSLKE SDRHTLLHFL EDEKYEEVMA VLGSFPYVTM DIKSQVLDDE DSNNITVGSL VTVLVKLTRQ TMAEVFEKEQ SICAAEEQPA EDGQGETNKN RTKGGWQQKS KGPKKTAKSK KKKPLKKKPT PVLLPQSKQQ KQKQANGVVG NEAAVKEDEE EVSDKGSDSE EEETNRDSQS EKDDGSDRDS DREQDEKQNK DDEAEWQELQ QSIQRKERAL LETKSKITHP VYSLYFPEEK QEWWWLYIAD RKEQTLISMP YHVCTLKDTE EVELKFPAPG KPGNYQYTVF LRSDSYMGLD QIKPLKLEVH EAKPVPENHP QWDTAIEGDE DQEDSEGFED SFEEEEEEEE DDD (409-760 aa encoded by BC047221)

Target Information

SEC63 gene ontology annotations related to this gene include liver development; multicellular organism aging; nitrogen compound metabolic process; posttranslational protein targeting to endoplasmic reticulum membrane; posttranslational protein targeting to membrane, translocation; renal system development; SRP-dependent cotranslational protein targeting to membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence.

The Journal of biological chemistry

Hirata T,Yang J,Tomida S,Tokoro Y,Kinoshita T,Fujita M,Kizuka Y

Sat Oct 01 00:00:00 UTC 2022

Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.

Journal of the American Society of Nephrology : JASN

Hassan H,Tian X,Inoue K,Chai N,Liu C,Soda K,Moeckel G,Tufro A,Lee AH,Somlo S,Fedeles S,Ishibe S

Fri Apr 01 00:00:00 UTC 2016

Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.

Journal of the American Society of Nephrology : JASN

Hassan H,Tian X,Inoue K,Chai N,Liu C,Soda K,Moeckel G,Tufro A,Lee AH,Somlo S,Fedeles S,Ishibe S

Fri Apr 01 00:00:00 UTC 2016

Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.

Journal of the American Society of Nephrology : JASN

Hassan H,Tian X,Inoue K,Chai N,Liu C,Soda K,Moeckel G,Tufro A,Lee AH,Somlo S,Fedeles S,Ishibe S

Fri Apr 01 00:00:00 UTC 2016

Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.

Journal of the American Society of Nephrology : JASN

Hassan H,Tian X,Inoue K,Chai N,Liu C,Soda K,Moeckel G,Tufro A,Lee AH,Somlo S,Fedeles S,Ishibe S

Fri Apr 01 00:00:00 UTC 2016

Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.

The Journal of clinical investigation

Fedeles SV,So JS,Shrikhande A,Lee SH,Gallagher AR,Barkauskas CE,Somlo S,Lee AH

Fri May 01 00:00:00 UTC 2015

Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.

The Journal of clinical investigation

Fedeles SV,So JS,Shrikhande A,Lee SH,Gallagher AR,Barkauskas CE,Somlo S,Lee AH

Fri May 01 00:00:00 UTC 2015

Cotranslational stabilization of Sec62/63 within the ER Sec61 translocon is controlled by distinct substrate-driven translocation events.

Molecular cell

Conti BJ,Devaraneni PK,Yang Z,David LL,Skach WR

Thu Apr 16 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: DnaJ homolog subfamily C member 23; SEC63 homolog; SEC63 protein translocation regulator; SEC63-like protein; Translocation protein SEC63 homolog

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Gene Aliases: 5730478J10Rik; AI649014; AW319215; DNAJC23; ERdj2; PRO2507; SEC63; SEC63L

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UniProt ID: (Human) Q9UGP8, (Mouse) Q8VHE0

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Entrez Gene ID: (Human) 11231, (Rat) 309858, (Mouse) 140740

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Function(s)

receptor activity protein binding poly(A) RNA binding protein transporter activity

Process(es)

liver development protein targeting to membrane SRP-dependent cotranslational protein targeting to membrane posttranslational protein targeting to membrane nitrogen compound metabolic process multicellular organism aging IRE1-mediated unfolded protein response renal system development posttranslational protein targeting to membrane, translocation transport protein transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SEC63 Polyclonal Antibody

SEC63 Antibody (13978-1-AP) in ICC/IF

Product Details

13978-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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