Proteintech

SECISBP2 Polyclonal Antibody

SECISBP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

SECISBP2 Antibody (12798-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 12798-1-AP (SECISBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

SECISBP2 Antibody in Immunoprecipitation (IP)

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Product Details

12798-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:20-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SECISBP2 Fusion Protein Ag3541 (506-854 aa encoded by BC036109)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: NPLDSSAPL MKKGKQREIP KAKKPTSLKK IILKERQERK QRLQENAVGP AFTSDDTQDG ESGGDDQFPE QAELSGPEGM DELISTPSVE DKSEEPPGTE LQRDTEASHL APNHTTFPKI HSRRFRDYCS QMLSKEVDAC VTDLLKELVR FQDRMYQKDP VKAKTKRRLV LGLREVLKHL KLKKLKCVII SPNCEKIQSK GGLDDTLHTI IDYACEQNIP FVFALNRKAL GRSLNKAVPV SVVGIFSYDG AQDQFHKMVE LTVAARQAYK TMLENVQQEL VGEPRPQAPP SLPTQGPSCP AEDGPPALKE KEEPHYIEIW KKHLEAYSGC TLELEESLEA STSQMMNLNL (506-854 aa encoded by BC036109)

Target Information

Eukaryotes require a selenocysteine (Sec) insertion sequence (SECIS) element in the 3' untranslated region of the mRNA to decode the UGA codon as Sec. SECIS-binding protein 2 (SBP-2) specifically binds selenoprotein mRNAs to form a functional complex and is essential for the insertion of Sec into selenoproteins. Purified SBP-2 interacts specifically with the SECIS element in the phospholipid hydroperoxide glutathione peroxidase mRNA. SBP-2 shows binding activity in the liver and testis as well as hepatoma cells. SBP-2 binds to a conserved RNA binding domain shared with several ribosomal proteins and eukaryotic translation termination release factor 1. A second domain located N-terminal to the RNA binding domain required for Sec insertion allows SBP-2 to stably associate with the ribosomal fraction of cells. SBP-2 preferentially stimulates incorporation directed by the selenoprotein P and phospholipid hydroperoxide glutathione peroxidase SECIS elements. SBP-2 may have a distinct function in selecting the ribosomes for Sec insertion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The expression of essential selenoproteins during development requires SECIS-binding protein 2-like.

Life science alliance

Kiledjian NT,Shah R,Vetick MB,Copeland PR

Sun May 01 00:00:00 UTC 2022

Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability.

Nature chemical biology

Li Z,Ferguson L,Deol KK,Roberts MA,Magtanong L,Hendricks JM,Mousa GA,Kilinc S,Schaefer K,Wells JA,Bassik MC,Goga A,Dixon SJ,Ingolia NT,Olzmann JA

Fri Jul 01 00:00:00 UTC 2022

Identification of a novel endogenous long non-coding RNA that inhibits selenoprotein P translation.

Nucleic acids research

Mita Y,Uchida R,Yasuhara S,Kishi K,Hoshi T,Matsuo Y,Yokooji T,Shirakawa Y,Toyama T,Urano Y,Inada T,Noguchi N,Saito Y

Fri Jul 09 00:00:00 UTC 2021

Role of the Thyroid Gland in Expression of the Thyroid Phenotype of Sbp2-Deficient Mice.

Endocrinology

Fujisawa H,Korwutthikulrangsri M,Fu J,Liao XH,Dumitrescu AM

Fri May 01 00:00:00 UTC 2020

Selenocysteine insertion sequence binding protein 2 (Sbp2) in the sex-specific regulation of selenoprotein gene expression in mouse pancreatic islets.

Scientific reports

Chellan B,Zhao L,Landeche M,Carmean CM,Dumitrescu AM,Sargis RM

Thu Oct 29 00:00:00 UTC 2020

Selenium-sensitive miRNA-181a-5p targeting SBP2 regulates selenoproteins expression in cartilage.

Journal of cellular and molecular medicine

Min Z,Guo Y,Sun M,Hussain S,Zhao Y,Guo D,Huang H,Heng L,Zhang F,Ning Q,Han Y,Xu P,Zhong N,Sun J,Lu S

Sat Dec 01 00:00:00 UTC 2018

Selenium-sensitive miRNA-181a-5p targeting SBP2 regulates selenoproteins expression in cartilage.

Journal of cellular and molecular medicine

Min Z,Guo Y,Sun M,Hussain S,Zhao Y,Guo D,Huang H,Heng L,Zhang F,Ning Q,Han Y,Xu P,Zhong N,Sun J,Lu S

Sat Dec 01 00:00:00 UTC 2018

Selenium-sensitive miRNA-181a-5p targeting SBP2 regulates selenoproteins expression in cartilage.

Journal of cellular and molecular medicine

Min Z,Guo Y,Sun M,Hussain S,Zhao Y,Guo D,Huang H,Heng L,Zhang F,Ning Q,Han Y,Xu P,Zhong N,Sun J,Lu S

Sat Dec 01 00:00:00 UTC 2018

Selenium-sensitive miRNA-181a-5p targeting SBP2 regulates selenoproteins expression in cartilage.

Journal of cellular and molecular medicine

Min Z,Guo Y,Sun M,Hussain S,Zhao Y,Guo D,Huang H,Heng L,Zhang F,Ning Q,Han Y,Xu P,Zhong N,Sun J,Lu S

Sat Dec 01 00:00:00 UTC 2018

Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.

Endocrinology

Fu J,Fujisawa H,Follman B,Liao XH,Dumitrescu AM

Fri Dec 01 00:00:00 UTC 2017

Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.

Endocrinology

Fu J,Fujisawa H,Follman B,Liao XH,Dumitrescu AM

Fri Dec 01 00:00:00 UTC 2017

Loss of selenocysteine insertion sequence binding protein 2 suppresses the proliferation, migration/invasion and hormone secretion of human trophoblast cells via the PI3K/Akt and ERK signaling pathway.

Placenta

Li M,Cheng W,Luo J,Hu X,Nie T,Lai H,Zheng X,Li F,Li H

Sat Jul 01 00:00:00 UTC 2017

Placenta

Li M,Cheng W,Luo J,Hu X,Nie T,Lai H,Zheng X,Li F,Li H

Sat Jul 01 00:00:00 UTC 2017

The Selenocysteine-Specific Elongation Factor Contains Unique Sequences That Are Required for Both Nuclear Export and Selenocysteine Incorporation.

PloS one

Dubey A,Copeland PR

Wed Jun 28 00:00:00 UTC 2017

SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding.

Science (New York, N.Y.)

Lin HC,Ho SC,Chen YY,Khoo KH,Hsu PH,Yen HC

Fri Jul 03 00:00:00 UTC 2015

SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding.

Science (New York, N.Y.)

Lin HC,Ho SC,Chen YY,Khoo KH,Hsu PH,Yen HC

Fri Jul 03 00:00:00 UTC 2015

Selective up-regulation of human selenoproteins in response to oxidative stress.

The Journal of biological chemistry

Touat-Hamici Z,Legrain Y,Bulteau AL,Chavatte L

Fri May 23 00:00:00 UTC 2014

Selective up-regulation of human selenoproteins in response to oxidative stress.

The Journal of biological chemistry

Touat-Hamici Z,Legrain Y,Bulteau AL,Chavatte L

Fri May 23 00:00:00 UTC 2014

Selective up-regulation of human selenoproteins in response to oxidative stress.

The Journal of biological chemistry

Touat-Hamici Z,Legrain Y,Bulteau AL,Chavatte L

Fri May 23 00:00:00 UTC 2014

Selective up-regulation of human selenoproteins in response to oxidative stress.

The Journal of biological chemistry

Touat-Hamici Z,Legrain Y,Bulteau AL,Chavatte L

Fri May 23 00:00:00 UTC 2014

Selective up-regulation of human selenoproteins in response to oxidative stress.

Free radical biology & medicine

Zahia TH,Yona L,Anne-Laure B,Laurent C

Wed Oct 01 00:00:00 UTC 2014

Selective up-regulation of human selenoproteins in response to oxidative stress.

Free radical biology & medicine

Zahia TH,Yona L,Anne-Laure B,Laurent C

Wed Oct 01 00:00:00 UTC 2014

Interplay between selenium levels, selenoprotein expression, and replicative senescence in WI-38 human fibroblasts.

The Journal of biological chemistry

Legrain Y,Touat-Hamici Z,Chavatte L

Fri Feb 28 00:00:00 UTC 2014

Interplay between selenium levels, selenoprotein expression, and replicative senescence in WI-38 human fibroblasts.

The Journal of biological chemistry

Legrain Y,Touat-Hamici Z,Chavatte L

Fri Feb 28 00:00:00 UTC 2014

Interplay between selenium levels, selenoprotein expression, and replicative senescence in WI-38 human fibroblasts.

The Journal of biological chemistry

Legrain Y,Touat-Hamici Z,Chavatte L

Fri Feb 28 00:00:00 UTC 2014

Interplay between selenium levels, selenoprotein expression, and replicative senescence in WI-38 human fibroblasts.

The Journal of biological chemistry

Legrain Y,Touat-Hamici Z,Chavatte L

Fri Feb 28 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: DKFZp686C09169; OTTHUMP00000064929; OTTHUMP00000064930; OTTHUMP00000064931; OTTHUMP00000064932; RP11-89K14.1; SECIS-binding protein 2; Selenocysteine insertion sequence-binding protein 2

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Gene Aliases: 2210413N07Rik; 2810012K13Rik; SBP2; SECISBP2

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UniProt ID: (Human) Q96T21, (Mouse) Q3U1C4, (Rat) Q9QX72

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Entrez Gene ID: (Human) 79048, (Mouse) 75420, (Rat) 79049

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Function(s)

mRNA 3'-UTR binding protein binding selenocysteine insertion sequence binding ribonucleoprotein complex binding poly(A) RNA binding RNA binding translation factor activity, RNA binding

Process(es)

selenocysteine incorporation striatum development neuron development negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SECISBP2 Polyclonal Antibody

SECISBP2 Antibody (12798-1-AP) in ICC/IF

Product Details

12798-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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