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SEMA3D Antibody (CF809917) in IHC (P)
Immunohistochemical staining of paraffin-embedded human embryonic brain cortex tissue within the normal limits using anti-SEMA3D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809917)(1:500)
Product Details
CF809917
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI2H4
Immunogen
Human recombinant protein fragment corresponding to amino acids 200-291 of human SEMA3D produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
SEMA3D induces the collapse and paralysis of neuronal growth cones. SEMA3D could potentially act as repulsive cues toward specific neuronal populations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: collapsin 2; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D; semaphorin 3D; semaphorin, homolog of the avian Sema3D; Semaphorin-3D
Gene Aliases: 4631426B19Rik; coll-2; Sema-Z2; SEMA3D; UNQ760/PRO1491
UniProt ID: (Human) O95025, (Mouse) Q8BH34
Entrez Gene ID: (Human) 223117, (Mouse) 108151, (Rat) 246262
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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