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Invitrogen
SEMA3E Polyclonal Antibody
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SEMA3E Antibody (PA5-47469) in IHC (F)
Immunohistochemical analysis of SEMA3E in immersion fixed frozen sections of adult mouse brain. Samples were incubated in SEMA3E polyclonal antibody (Product # PA5-47469) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cerebellum.
Product Details
PA5-47469
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3E Thr25-Ser775 (Arg557Ala and Arg560Ala)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610392
Product Specific Information
In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) Semaphorin 3E is observed and less than 10% cross-reactivity with rmSemaphorin 3A, rmSemaphorin 3B, rmSemaphorin 3C, rmSemaphorin 3F, and recombinant human (rh) Semaphorin 3A, rhSemaphorin 3C, rhSemaphorin 3D, and rhSemaphorin 3F is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
The Semaphorins make up the largest family of axon guidance cues yet described. Semaphorins are divided into 8 classes (classes 3-7 found in vertebrates). Class 3 Semaphorins are secreted, classes 4 through 6 are transmembrane proteins, and class 7 are membrane associated via glycosylphosphatidylinositol (GPI) linkage. They are characterized structurally by a conserved approximately 400 amino acid sema domain. They are classically described as collapsing factors and mediators of axon repulsion, although they may also act as context-dependent chemoattractants. Semaphorins have been shown to have roles in cardiovascular development and in the regulation of immune cell antigen presentation. Receptors or receptor complexes that mediate semaphorin signaling include proteins of the Neuropilin and Plexin families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: M-sema H; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; Sema H; Semaphorin-3E; Semaphorin-H
Gene Aliases: 6430702L12; AA408817; coll-5; KIAA0331; M-SEMAH; M-SemaK; mKIAA0331; SEMA3E; SEMAH; Semh
UniProt ID: (Human) O15041, (Mouse) P70275
Entrez Gene ID: (Human) 9723, (Mouse) 20349
angiogenesis
patterning of blood vessels
negative regulation of cell-matrix adhesion
sprouting angiogenesis
multicellular organism development
nervous system development
regulation of cell shape
negative regulation of angiogenesis
cell differentiation
synapse organization
semaphorin-plexin signaling pathway
regulation of actin cytoskeleton reorganization
neural crest cell migration
positive regulation of cell migration
negative regulation of axon extension involved in axon guidance
negative chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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