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Invitrogen
SEMA4C Polyclonal Antibody
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FIGURE: 1 / 5
SEMA4C Antibody (PA5-47812) in ICC/IF
Immunocytochemical analysis of Semaphorin 4C was detected in immersion fixed rat cortical stem cells using Sheep Anti-mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47812) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
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.jpg?time=20250104?time=20250104 "SEMA4C Antibody (PA5-47812) in IHC (F)")
Product Details
PA5-47812
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4C Glu22-Gly664
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607332
Product Specific Information
In direct ELISAs, approximately 20% cross-reactivity with recombinant human Semaphorin 4C and less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4F, and Semaphorin 4G is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
SEMA4C (semaphorin-4C), also known as SEMAF, is an 833 amino acid single-pass type I membrane protein that contains one sema domain, one PSI domain and one Ig-like C2-type domain. Expressed in a variety of tissues, including lung, kidney and brain, SEMA4C interacts with GIPC and NCDN and is thought to play a role in the formation of neural networks during development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ20369; M-Sema F; MGC126382; MGC126383; Sema I; Semaphorin-4C; Semaphorin-C-like 1
Gene Aliases: AI426163; M-Sema F; RGD1562837; S4c; sema I; Sema4c; Semacl1; Semaf; Semai
UniProt ID: (Mouse) Q64151
Entrez Gene ID: (Rat) 301346, (Mouse) 20353
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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