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Invitrogen
SEMA5B Polyclonal Antibody
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SEMA5B Antibody (PA5-145463) in IHC (P)
Immunohistochemistry (Paraffin) analysis of SEMA5B in Human Cardiac Muscle Tissue. Samples were incubated in SEMA5B polyclonal antibody (Product # PA5-145463) using a dilution of 20 µg/mL followed by HRP-Linked Caprine Anti-Rabbit IgG at a dilution of 2 µg/mL and DAB staining.
Product Details
PA5-145463
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human SEMA5B protein, Leu36-Pro602 (Accession #Q9P283)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.05% Proclin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3093620
Target Information
Semaphorin 5B (SEMA5B) is a gene belonging to the Semaphorin family, which is known for its role as guidance cues in various biological processes. The gene is located on chromosome 3q21.3 and encodes for the transmembrane protein Semaphorin 5B, which participates significantly in neural development and cancer processes. SEMA5B serves as a guidance cue in neural tissues, impacting axonal growth and branching by acting through receptors like PlexinA1. It is proteolytically processed from its transmembrane form to a soluble form, capable of collapsing growth cones in neurons, thereby regulating neuronal pathfinding. Studies have revealed that SEMA5B impacts sensory afferent projections through interactions with cell adhesion molecules such as TAG-1. Moreover, SEMA5B has been implicated in cancer, particularly showing upregulated expression in kidney renal clear cell carcinoma (KIRC) at both mRNA and protein levels. Its expression correlates with various clinical parameters, including tumor stage and patient demographics. Its dynamic expression pattern in the spinal cord suggests a role in the modulation of sensory axon growth, further emphasizing its importance in developmental neurobiology and pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B; Sema G; semaphorin 5B; Semaphorin-5B; Semaphorin-G
Gene Aliases: AI893641; KIAA1445; mKIAA1445; SEMA5B; SEMAG; SemG; UNQ5867/PRO34001
UniProt ID: (Human) Q9P283, (Mouse) Q60519
Entrez Gene ID: (Human) 54437, (Rat) 303901, (Mouse) 20357
multicellular organism development
nervous system development
cell differentiation
neural crest cell migration
positive regulation of cell migration
axon extension
negative regulation of axon extension involved in axon guidance
detection of light stimulus involved in visual perception
negative chemotaxis
semaphorin-plexin signaling pathway
neuron projection guidance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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