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Invitrogen
SEMA6A Polyclonal Antibody
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FIGURE: 1 / 2
SEMA6A Antibody (PA5-81009) in ICC/IF
Immunofluorescence staining of SEMA6A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with SEMA6A Polyclonal Antibody (Product # PA5-81009, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Product Details
PA5-81009
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human SEMA6A Protein (Met1-Thr649)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788265
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SEMA6A.
Target Information
SEMA6A can act as repulsive axon guidance cues. It may play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA6A-1; semaphorin 6A-1; Semaphorin VIA; Semaphorin-6A; Semaphorin-6A-1
Gene Aliases: HT018; KIAA1368; SEMA; SEMA6A; SEMA6A1; SEMAQ; VIA
UniProt ID: (Human) Q9H2E6
Entrez Gene ID: (Human) 57556
neural crest cell migration
apoptotic process
cytoskeleton organization
cell surface receptor signaling pathway
nervous system development
axon guidance
organ morphogenesis
negative regulation of axon extension involved in axon guidance
negative chemotaxis
semaphorin-plexin signaling pathway
positive regulation of neuron migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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