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SENP8 Antibody (PA5-81543) in ICC/IF
Immunofluorescence staining of SENP8 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with SENP8 Polyclonal Antibody (Product # PA5-81543, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-81543
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human SENP8
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788746
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human SENP8.
Target Information
SENP8 (Cysteine protease FKSG8; DEN1; FKSG8; HsT17512; NEDP1; PRSC2; SUMO/sentrin specific protease family member 8), is a novel cysteine protease which belongs to the ULP family of deubiquitinases. ULP family members consist of a C-terminal core catalytic domain and N-terminal extensions determining substrate specificity. SENP8, a 24KDa protein, plays a major role in regulating NEDD8 pathway. It processes preNEDD8 to its mature form and deconjugates NEDD8 from substrates such as Cullins and p53. SENP8 may play a role in preventing accumulation of hyper-neddylated cullin and maintaining the level of mono-neddylated cullin that is thought to increase the efficiency of ubiquitination by SCF ubiquitin ligases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: deneddylase 1; Deneddylase-1; NEDD8 specific-protease cysteine 2; NEDD8-specific protease 1; Protease, cysteine 2; Sentrin-specific protease 8; Sentrin/SUMO-specific protease SENP8; SUMO sentrin specific protease family member 8; SUMO/sentrin specific peptidase family member 8
Gene Aliases: DEN1; FKSG8; NEDP1; PRSC2; SENP8
UniProt ID: (Human) Q96LD8
Entrez Gene ID: (Human) 123228
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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