Proteintech

SEPT2 Monoclonal Antibody (2F3B2)

SEPT2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

SEPT2 Antibody (60075-1-IG) in ICC/IF

IF result of anti-SEPT2 (60075-1-IG, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: SEPT2. Blue: DNA. 1% PFA fixed.

SEPT2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

60075-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000-1:200,000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:2000

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F3B2

Immunogen

SEPT2 Fusion Protein Ag1946 (1-361 aa encoded by BC014455)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSKQQPTQF INPETPGYVG FANLPNQVHR KSVKKGFEFT LMVVGESGLG KSTLINSLFL TDLYPERVIS GAAEKIERTV QIEASTVEIE ERGVKLRLTV VDTPGYGDAI NCRDCFKTII SYIDEQFERY LHDESGLNRR HIIDNRVHCC FYFISPFGHG LKPLDVAFMK AIHNKVNIVP VIAKADTLTL KERERLKKRI LDEIEEHNIK IYHLPDAESD EDEDFKEQTR LLKASIPFSV VGSNQLIEAK GKKVRGRLYP WGVVEVENPE HNDFLKLRTM LITHMQDLQE VTQDLHYENF RSERLKRGGR KVENEDMNKD QILLEKEAEL RRMQEMIARM QAQMQMQMQG GDGDGGALGH HV (1-361 aa encoded by BC014455)

Target Information

Septin 2 is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton and plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. It is required for the progression through mitosis, and it forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, Septin 2 may be required for chromosome segregation and spindle elongation. Septin 2 also plays a role in ciliogenesis and collective cell movements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.

The Journal of cell biology

Martins CS,Taveneau C,Castro-Linares G,Baibakov M,Buzhinsky N,Eroles M,Milanović V,Omi S,Pedelacq JD,Iv F,Bouillard L,Llewellyn A,Gomes M,Belhabib M,Kuzmić M,Verdier-Pinard P,Lee S,Badache A,Kumar S,Chandre C,Brasselet S,Rico F,Rossier O,Koenderink GH,Wenger J,Cabantous S,Mavrakis M

Mon Mar 06 00:00:00 UTC 2023

Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.

The Journal of cell biology

Mon Mar 06 00:00:00 UTC 2023

Splenectomy improves erythrocyte functionality in spherocytosis based on septin abundance, but not maturation defects.

Blood advances

Cloos AS,Pollet H,Stommen A,Maja M,Lingurski M,Brichard B,Lambert C,Henriet P,Pierreux C,Pyr Dit Ruys S,Van Der Smissen P,Vikkula M,Gatto L,Martin M,Brouillard P,Vertommen D,Tyteca D

Tue Sep 12 00:00:00 UTC 2023

Septins promote caspase activity and coordinate mitochondrial apoptosis.

Cytoskeleton (Hoboken, N.J.)

Van Ngo H,Robertin S,Brokatzky D,Bielecka MK,Lobato-Márquez D,Torraca V,Mostowy S

Fri Mar 22 00:00:00 UTC 2024

Orai1 is an Entotic Ca²⁺ Channel for Non-Apoptotic Cell Death, Entosis in Cancer Development.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Lee AR,Park CY

Mon May 01 00:00:00 UTC 2023

Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets.

Molecular & cellular proteomics : MCP

Tshilenge KT,Aguirre CG,Bons J,Gerencser AA,Basisty N,Song S,Rose J,Lopez-Ramirez A,Naphade S,Loureiro A,Battistoni E,Milani M,Wehrfritz C,Holtz A,Hetz C,Mooney SD,Schilling B,Ellerby LM

Mon May 01 00:00:00 UTC 2023

Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.

Cell research

Wang L,Paudyal SC,Kang Y,Owa M,Liang FX,Spektor A,Knaut H,Sánchez I,Dynlacht BD

Tue Feb 01 00:00:00 UTC 2022

Contribution of septins to human platelet structure and function.

iScience

Kim OV,Litvinov RI,Mordakhanova ER,Bi E,Vagin O,Weisel JW

Fri Jul 15 00:00:00 UTC 2022

Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.

Neuron

Li H,Saucedo-Cuevas L,Yuan L,Ross D,Johansen A,Sands D,Stanley V,Guemez-Gamboa A,Gregor A,Evans T,Chen S,Tan L,Molina H,Sheets N,Shiryaev SA,Terskikh AV,Gladfelter AS,Shresta S,Xu Z,Gleeson JG

Wed Mar 20 00:00:00 UTC 2019

Zika Virus Protease Cleavage of Host Protein Septin-2 Mediates Mitotic Defects in Neural Progenitors.

Neuron

Li H,Saucedo-Cuevas L,Yuan L,Ross D,Johansen A,Sands D,Stanley V,Guemez-Gamboa A,Gregor A,Evans T,Chen S,Tan L,Molina H,Sheets N,Shiryaev SA,Terskikh AV,Gladfelter AS,Shresta S,Xu Z,Gleeson JG

Wed Mar 20 00:00:00 UTC 2019

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Cytoskeleton (Hoboken, N.J.)

Lobato-Márquez D,Krokowski S,Sirianni A,Larrouy-Maumus G,Mostowy S

Tue Jan 01 00:00:00 UTC 2019

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Cytoskeleton (Hoboken, N.J.)

Lobato-Márquez D,Krokowski S,Sirianni A,Larrouy-Maumus G,Mostowy S

Tue Jan 01 00:00:00 UTC 2019

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Cytoskeleton (Hoboken, N.J.)

Lobato-Márquez D,Krokowski S,Sirianni A,Larrouy-Maumus G,Mostowy S

Tue Jan 01 00:00:00 UTC 2019

A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.

Cytoskeleton (Hoboken, N.J.)

Lobato-Márquez D,Krokowski S,Sirianni A,Larrouy-Maumus G,Mostowy S

Tue Jan 01 00:00:00 UTC 2019

Septins suppress the release of vaccinia virus from infected cells.

The Journal of cell biology

Pfanzelter J,Mostowy S,Way M

Mon Aug 06 00:00:00 UTC 2018

Septins suppress the release of vaccinia virus from infected cells.

The Journal of cell biology

Pfanzelter J,Mostowy S,Way M

Mon Aug 06 00:00:00 UTC 2018

Cdc42 regulates Cdc42EP3 function in cancer-associated fibroblasts.

Small GTPases

Farrugia AJ,Calvo F

Mon Jan 02 00:00:00 UTC 2017

Cdc42 regulates Cdc42EP3 function in cancer-associated fibroblasts.

Small GTPases

Farrugia AJ,Calvo F

Mon Jan 02 00:00:00 UTC 2017

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Cell reports

Calvo F,Ranftl R,Hooper S,Farrugia AJ,Moeendarbary E,Bruckbauer A,Batista F,Charras G,Sahai E

Tue Dec 29 00:00:00 UTC 2015

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Cell reports

Calvo F,Ranftl R,Hooper S,Farrugia AJ,Moeendarbary E,Bruckbauer A,Batista F,Charras G,Sahai E

Tue Dec 29 00:00:00 UTC 2015

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Cell reports

Calvo F,Ranftl R,Hooper S,Farrugia AJ,Moeendarbary E,Bruckbauer A,Batista F,Charras G,Sahai E

Tue Dec 29 00:00:00 UTC 2015

Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.

Cell reports

Calvo F,Ranftl R,Hooper S,Farrugia AJ,Moeendarbary E,Bruckbauer A,Batista F,Charras G,Sahai E

Tue Dec 29 00:00:00 UTC 2015

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 UTC 2011

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Nature genetics

Garcia-Gonzalo FR,Corbit KC,Sirerol-Piquer MS,Ramaswami G,Otto EA,Noriega TR,Seol AD,Robinson JF,Bennett CL,Josifova DJ,García-Verdugo JM,Katsanis N,Hildebrandt F,Reiter JF

Sun Jul 03 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 2-Sep; NEDD-5; Neural precursor cell expressed developmentally down-regulated protein 5; neural precursor cell expressed, developmentally down-regulated 5; neural precursor cell expressed, developmentally down-regulated gene 5; Septin-2; Vascular endothelial cell specific protein 11

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Gene Aliases: AW208991; DIFF6; hNedd5; KIAA0158; mKIAA0158; NEDD-5; NEDD5; Pnutl3; SEPT2; SEPTIN2; Vesp11

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UniProt ID: (Human) Q15019, (Mouse) P42208, (Rat) Q91Y81

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Entrez Gene ID: (Human) 4735, (Mouse) 18000, (Rat) 117515

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Function(s)

protein binding GTP binding enzyme regulator activity protein complex scaffold cadherin binding involved in cell-cell adhesion nucleotide binding GTPase activity identical protein binding

Process(es)

cytokinesis regulation of L-glutamate transport cell cycle mitotic nuclear division smoothened signaling pathway neuron projection development regulation of protein localization cilium assembly regulation of catalytic activity protein polymerization cell division metabolic process cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SEPT2 Monoclonal Antibody (2F3B2)

SEPT2 Antibody (60075-1-IG) in ICC/IF

Product Details

60075-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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