Proteintech

SEPT9 Polyclonal Antibody

SEPT9 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

SEPT9 Antibody (10769-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using SEPT9 antibody 10769-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SEPT9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SEPT9 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10769-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 15 publications 15 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SEPT9 Fusion Protein Ag1218 (250-568 aa encoded by BC021192)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RNEKAPVDF GYVGIDSILE QMRRKAMKQG FEFNIMVVGQ SGLGKSTLIN TLFKSKISRK SVQPTSEERI PKTIEIKSIT HDIEEKGVRM KLTVIDTPGF GDHINNENCW QPIMKFINDQ YEKYLQEEVN INRKKRIPDT RVHCCLYFIP ATGHSLRPLD IEFMKRLSKV VNIVPVIAKA DTLTLEERVH FKQRITADLL SNGIDVYPQK EFDEDSEDRL VNEKFREMIP FAVVGSDHEY QVNGKRILGR KTKWGTIEVE NTTHCEFAYL RDLLIRTHMQ NIKDITSSIH FEAYRVKRLN EGSSAMANGV EEKEPEAPEM (250-568 aa encoded by BC021192)

Target Information

The maf oncogene was identified by structural analysis of the AS42 avian transforming retrovirus genome. The Maf family is divided into two subclasses, large Mafs (vMaf, cMaf, MafB and Nrl) and small Mafs (MafF, MafK, and MafG). Both subclasses contain leucinezipper motifs, which allow homodimerization as well as heterodimerization with a variety of other bZip transcription factors. Large Mafs also contain an acidic transactivation domain absent in the small Maf proteins. Although they do not possess inherent transactivation activity, small Maf proteins can act as positive regulators of transcription by targeting transcriptionally active dimerization partners to specific DNA regulatory elements. Conversely, small Mafs can act also as negative regulators of transcription by recruiting transcriptional repressors or by forming homodimers that can replace active dimers. Human MafF was isolated in a yeast one-hybrid system from a human myometrium cDNA library. Human MAFF encodes a 164 amino acids proten. Like other small MAFF proteins, it contains an extended leucine zipper structure and lacks an N-terminal transactivating domain. The three small Maf proteins have been implicated in a number of physiological processes, including development, differentiation, haematopoiesis and stress response. Interestingly, these three proteins regulate the stress response via different mechanisms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.

The Journal of cell biology

Martins CS,Taveneau C,Castro-Linares G,Baibakov M,Buzhinsky N,Eroles M,Milanović V,Omi S,Pedelacq JD,Iv F,Bouillard L,Llewellyn A,Gomes M,Belhabib M,Kuzmić M,Verdier-Pinard P,Lee S,Badache A,Kumar S,Chandre C,Brasselet S,Rico F,Rossier O,Koenderink GH,Wenger J,Cabantous S,Mavrakis M

Mon Mar 06 00:00:00 UTC 2023

Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.

The Journal of cell biology

Mon Mar 06 00:00:00 UTC 2023

Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice.

Cytoskeleton (Hoboken, N.J.)

Martens AK,Erwig M,Patzig J,Fledrich R,Füchtbauer EM,Werner HB

Tue Sep 19 00:00:00 UTC 2023

Targeted inactivation of the Septin2 and Septin9 genes in myelinating Schwann cells of mice.

Cytoskeleton (Hoboken, N.J.)

Martens AK,Erwig M,Patzig J,Fledrich R,Füchtbauer EM,Werner HB

Tue Sep 19 00:00:00 UTC 2023

Proteomic Analysis of Huntington's Disease Medium Spiny Neurons Identifies Alterations in Lipid Droplets.

Molecular & cellular proteomics : MCP

Tshilenge KT,Aguirre CG,Bons J,Gerencser AA,Basisty N,Song S,Rose J,Lopez-Ramirez A,Naphade S,Loureiro A,Battistoni E,Milani M,Wehrfritz C,Holtz A,Hetz C,Mooney SD,Schilling B,Ellerby LM

Mon May 01 00:00:00 UTC 2023

Septin-microtubule association via a motif unique to isoform 1 of septin 9 tunes stress fibers.

Journal of cell science

Kuzmić M,Castro Linares G,Leischner Fialová J,Iv F,Salaün D,Llewellyn A,Gomes M,Belhabib M,Liu Y,Asano K,Rodrigues M,Isnardon D,Tachibana T,Koenderink GH,Badache A,Mavrakis M,Verdier-Pinard P

Sat Jan 01 00:00:00 UTC 2022

An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis.

Cell reports

Panagiotou TC,Chen A,Wilde A

Tue Aug 30 00:00:00 UTC 2022

An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis.

Cell reports

Panagiotou TC,Chen A,Wilde A

Tue Aug 30 00:00:00 UTC 2022

SEPT9 Upregulation in Satellite Glial Cells Associated with Diabetic Polyneuropathy in a Type 2 Diabetes-like Rat Model.

International journal of molecular sciences

Kan HW,Ho YC,Chang YS,Hsieh YL

Fri Aug 19 00:00:00 UTC 2022

DCHS1, Lix1L, and the Septin Cytoskeleton: Molecular and Developmental Etiology of Mitral Valve Prolapse.

Journal of cardiovascular development and disease

Moore KS,Moore R,Fulmer DB,Guo L,Gensemer C,Stairley R,Glover J,Beck TC,Morningstar JE,Biggs R,Muhkerjee R,Awgulewitsch A,Norris RA

Thu Feb 17 00:00:00 UTC 2022

Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.

Current biology : CB

Salameh J,Cantaloube I,Benoit B,Poüs C,Baillet A

Mon Sep 27 00:00:00 UTC 2021

Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.

Current biology : CB

Salameh J,Cantaloube I,Benoit B,Poüs C,Baillet A

Mon Sep 27 00:00:00 UTC 2021

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

The Journal of cell biology

Kesisova IA,Robinson BP,Spiliotis ET

Mon Feb 01 00:00:00 UTC 2021

A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.

The Journal of cell biology

Kesisova IA,Robinson BP,Spiliotis ET

Mon Feb 01 00:00:00 UTC 2021

Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.

Molecular biology of the cell

Soroor F,Kim MS,Palander O,Balachandran Y,Collins RF,Benlekbir S,Rubinstein JL,Trimble WS

Mon Feb 01 00:00:00 UTC 2021

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.

Journal of cell science

Iv F,Martins CS,Castro-Linares G,Taveneau C,Barbier P,Verdier-Pinard P,Camoin L,Audebert S,Tsai FC,Ramond L,Llewellyn A,Belhabib M,Nakazawa K,Di Cicco A,Vincentelli R,Wenger J,Cabantous S,Koenderink GH,Bertin A,Mavrakis M

Sun Aug 01 00:00:00 UTC 2021

Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.

Oncogene

Marcus J,Bejerano-Sagie M,Patterson N,Bagchi S,Verkhusha VV,Connolly D,Goldberg GL,Golden A,Sharma VP,Condeelis J,Montagna C

Mon Jul 01 00:00:00 UTC 2019

Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.

Oncogene

Marcus J,Bejerano-Sagie M,Patterson N,Bagchi S,Verkhusha VV,Connolly D,Goldberg GL,Golden A,Sharma VP,Condeelis J,Montagna C

Mon Jul 01 00:00:00 UTC 2019

Characterization of septin expression in normal and fibrotic kidneys.

Cytoskeleton (Hoboken, N.J.)

Neubauer K,Neubauer B,Seidl M,Zieger B

Tue Jan 01 00:00:00 UTC 2019

Characterization of septin expression in normal and fibrotic kidneys.

Cytoskeleton (Hoboken, N.J.)

Neubauer K,Neubauer B,Seidl M,Zieger B

Tue Jan 01 00:00:00 UTC 2019

A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.

Current biology : CB

Karasmanis EP,Hwang D,Nakos K,Bowen JR,Angelis D,Spiliotis ET

Mon Jul 08 00:00:00 UTC 2019

Septin filament coalignment with microtubules depends on SEPT9_i1 and tubulin polyglutamylation, and is an early feature of acquired cell resistance to paclitaxel.

Cell death & disease

Targa B,Klipfel L,Cantaloube I,Salameh J,Benoit B,Poüs C,Baillet A

Tue Jan 22 00:00:00 UTC 2019

Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.

Developmental cell

Karasmanis EP,Phan CT,Angelis D,Kesisova IA,Hoogenraad CC,McKenney RJ,Spiliotis ET

Mon Jul 16 00:00:00 UTC 2018

Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.

Developmental cell

Karasmanis EP,Phan CT,Angelis D,Kesisova IA,Hoogenraad CC,McKenney RJ,Spiliotis ET

Mon Jul 16 00:00:00 UTC 2018

Septins suppress the release of vaccinia virus from infected cells.

The Journal of cell biology

Pfanzelter J,Mostowy S,Way M

Mon Aug 06 00:00:00 UTC 2018

Septins suppress the release of vaccinia virus from infected cells.

The Journal of cell biology

Pfanzelter J,Mostowy S,Way M

Mon Aug 06 00:00:00 UTC 2018

Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.

Journal of cell science

Danson CM,Pearson N,Heesom KJ,Cullen PJ

Mon Sep 10 00:00:00 UTC 2018

Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.

Journal of cell science

Danson CM,Pearson N,Heesom KJ,Cullen PJ

Mon Sep 10 00:00:00 UTC 2018

Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.

Journal of cell science

Danson CM,Pearson N,Heesom KJ,Cullen PJ

Mon Sep 10 00:00:00 UTC 2018

Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.

Journal of cell science

Danson CM,Pearson N,Heesom KJ,Cullen PJ

Mon Sep 10 00:00:00 UTC 2018

Septins are critical regulators of osteoclastic bone resorption.

Scientific reports

Møller AMJ,Füchtbauer EM,Brüel A,Andersen TL,Borggaard XG,Pavlos NJ,Thomsen JS,Pedersen FS,Delaisse JM,Søe K

Wed Aug 29 00:00:00 UTC 2018

Septins are critical regulators of osteoclastic bone resorption.

Scientific reports

Møller AMJ,Füchtbauer EM,Brüel A,Andersen TL,Borggaard XG,Pavlos NJ,Thomsen JS,Pedersen FS,Delaisse JM,Søe K

Wed Aug 29 00:00:00 UTC 2018

Septins are critical regulators of osteoclastic bone resorption.

Scientific reports

Møller AMJ,Füchtbauer EM,Brüel A,Andersen TL,Borggaard XG,Pavlos NJ,Thomsen JS,Pedersen FS,Delaisse JM,Søe K

Wed Aug 29 00:00:00 UTC 2018

Septins are critical regulators of osteoclastic bone resorption.

Scientific reports

Møller AMJ,Füchtbauer EM,Brüel A,Andersen TL,Borggaard XG,Pavlos NJ,Thomsen JS,Pedersen FS,Delaisse JM,Søe K

Wed Aug 29 00:00:00 UTC 2018

Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.

Molecular biology of the cell

Bai X,Karasmanis EP,Spiliotis ET

Tue Mar 15 00:00:00 UTC 2016

Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.

Molecular biology of the cell

Bai X,Karasmanis EP,Spiliotis ET

Tue Mar 15 00:00:00 UTC 2016

Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.

Molecular biology of the cell

Bai X,Karasmanis EP,Spiliotis ET

Tue Mar 15 00:00:00 UTC 2016

Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.

Molecular biology of the cell

Bai X,Karasmanis EP,Spiliotis ET

Tue Mar 15 00:00:00 UTC 2016

Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cells.

Methods in cell biology

Spiliotis ET,Karasmanis EP,Dolat L

Mon Aug 21 00:00:00 UTC 2017

Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cells.

Methods in cell biology

Spiliotis ET,Karasmanis EP,Dolat L

Mon Aug 21 00:00:00 UTC 2017

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.

PLoS genetics

Menon MB,Sawada A,Chaturvedi A,Mishra P,Schuster-Gossler K,Galla M,Schambach A,Gossler A,Förster R,Heuser M,Kotlyarov A,Kinoshita M,Gaestel M

Fri Aug 01 00:00:00 UTC 2014

Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.

PLoS genetics

Menon MB,Sawada A,Chaturvedi A,Mishra P,Schuster-Gossler K,Galla M,Schambach A,Gossler A,Förster R,Heuser M,Kotlyarov A,Kinoshita M,Gaestel M

Fri Aug 01 00:00:00 UTC 2014

Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Patzig J,Jahn O,Tenzer S,Wichert SP,de Monasterio-Schrader P,Rosfa S,Kuharev J,Yan K,Bormuth I,Bremer J,Aguzzi A,Orfaniotou F,Hesse D,Schwab MH,Möbius W,Nave KA,Werner HB

Wed Nov 09 00:00:00 UTC 2011

Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Patzig J,Jahn O,Tenzer S,Wichert SP,de Monasterio-Schrader P,Rosfa S,Kuharev J,Yan K,Bormuth I,Bremer J,Aguzzi A,Orfaniotou F,Hesse D,Schwab MH,Möbius W,Nave KA,Werner HB

Wed Nov 09 00:00:00 UTC 2011

Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Patzig J,Jahn O,Tenzer S,Wichert SP,de Monasterio-Schrader P,Rosfa S,Kuharev J,Yan K,Bormuth I,Bremer J,Aguzzi A,Orfaniotou F,Hesse D,Schwab MH,Möbius W,Nave KA,Werner HB

Wed Nov 09 00:00:00 UTC 2011

Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Patzig J,Jahn O,Tenzer S,Wichert SP,de Monasterio-Schrader P,Rosfa S,Kuharev J,Yan K,Bormuth I,Bremer J,Aguzzi A,Orfaniotou F,Hesse D,Schwab MH,Möbius W,Nave KA,Werner HB

Wed Nov 09 00:00:00 UTC 2011

Deciphering the rules governing assembly order of mammalian septin complexes.

Molecular biology of the cell

Sellin ME,Sandblad L,Stenmark S,Gullberg M

Thu Sep 01 00:00:00 UTC 2011

Deciphering the rules governing assembly order of mammalian septin complexes.

Molecular biology of the cell

Sellin ME,Sandblad L,Stenmark S,Gullberg M

Thu Sep 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: E-septin; Eighth septin; Eseptin; MLL septin-like fusion; MLL septin-like fusion protein; MLL septin-like fusion protein MSF-A; Ov/Br septin; Ovarian/Breast septin; Septin D1; Septin-9; Septin-like protein; SL3-3 integration site 1 protein; SLP

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Gene Aliases: AF17q25; Eseptin; KIAA0991; MSF; MSF1; NAPB; PNUTL4; SEPT9; SeptD1; SEPTIN9; SINT1; Slpa

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UniProt ID: (Human) Q9UHD8, (Rat) Q9QZR6, (Mouse) Q80UG5

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Entrez Gene ID: (Human) 10801, (Rat) 83788, (Mouse) 53860

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Function(s)

GTPase activity protein binding GTP binding cadherin binding involved in cell-cell adhesion molecular_function nucleotide binding

Process(es)

cell cycle protein heterooligomerization cell division cell-cell adhesion positive regulation of nonmotile primary cilium assembly biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SEPT9 Polyclonal Antibody

SEPT9 Antibody (10769-1-AP) in ICC/IF

Product Details

10769-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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