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          • Primary Antibodies ›
          • SERCA1 ATPase Antibodies

          Invitrogen

          SERCA1 ATPase Monoclonal Antibody (IIH11)

          19 Published Figures
          78 References
          View all (23) SERCA1 ATPase antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite SERCA1 ATPase Monoclonal Antibody (IIH11)

          • Antibody Testing Data (6)
          • Published Figures (19)
          SERCA1 ATPase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SERCA1 ATPase Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 25

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          SERCA1 ATPase Antibody (MA3-911) in ICC/IF

          Immunofluorescent analysis of SERCA1 ATPase using Anti-SERCA1 ATPase Monoclonal Antibody (IIH11) (Product # MA3-911) shows staining in C6 Cells. SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SER... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          SERCA1 ATPase Antibody in Immunocytochemistry (ICC/IF)
          SERCA1 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SERCA1 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SERCA1 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SERCA1 ATPase Antibody in Immunohistochemistry (IHC)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Immunohistochemistry (IHC)
          SERCA1 ATPase Antibody in Immunohistochemistry (IHC)
          SERCA1 ATPase Antibody in Immunohistochemistry (IHC)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)
          SERCA1 ATPase Antibody in Western Blot (WB)

          Product Details

          MA3-911

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:5,000
          View 60 publications 60 publications

          Immunohistochemistry (IHC)

          -
          View 16 publications 16 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:500
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Dog, Guinea pig, Human, Mouse, Rabbit, Rat

          Published species

          Dog, Human, Invertebrate, Mouse, Rabbit, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          IIH11

          Immunogen

          Purified rabbit skeletal muscle sarcoplasmic reticulum.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          PBS, ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325494

          Product Specific Information

          MA3-911 detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase from canine, human, rabbit, rat, mouse and guinea pig tissues.

          MA3-911 has been successfully used in Western blot, immunohistochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~110 kDa protein representing SERCA1 ATPase in canine skeletal muscle extracts. Immunofluorescence staining of SERCA1 ATPase in canine skeletal muscle with MA3-911 results in strong labeling of the entire type II (fast) myofiber.

          The MA3-911 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum. This antibody recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

          Target Information

          ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, ubiquitous; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; SERCA1; SR Ca(2+)-ATPase 1

          View more View less

          Gene Aliases: ATP2A; ATP2A1; ATP2A3; SERCA1; SERCA1a

          View more View less

          UniProt ID: (Human) O14983, (Rabbit) P04191, (Mouse) Q8R429, (Rat) Q64578

          View more View less

          Entrez Gene ID: (Dog) 479797, (Human) 487, (Rabbit) 100037716, (Mouse) 11937, (Rat) 116601

          View more View less

          Function(s)
          calcium-transporting ATPase activity calcium ion binding protein binding ATP binding protein homodimerization activity nucleotide binding nucleotide-sugar transmembrane transporter activity hydrolase activity metal ion binding
          Process(es)
          calcium ion transport apoptotic mitochondrial changes positive regulation of fast-twitch skeletal muscle fiber contraction positive regulation of endoplasmic reticulum calcium ion concentration negative regulation of endoplasmic reticulum calcium ion concentration ion transmembrane transport response to endoplasmic reticulum stress positive regulation of mitochondrial calcium ion concentration maintenance of mitochondrion location intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress calcium ion import calcium ion transmembrane transport relaxation of skeletal muscle regulation of cardiac conduction transport ion transport regulation of muscle contraction regulation of striated muscle contraction response to peptide hormone negative regulation of striated muscle contraction sarcoplasmic reticulum calcium ion transport carbohydrate derivative transport nucleotide transmembrane transport metabolic process
          It has to be done as per old AB suggested Products section.
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