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Invitrogen
SERCA2 ATPase Recombinant Rabbit Monoclonal Antibody (20D1)
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FIGURE: 1 / 3
SERCA2 ATPase Antibody (MA5-50156) in ICC/IF
Immunocytochemistry analysis of MCF7 with SERCA2 ATPase monoclonal antibody (Product # MA5-50156) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 509-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Product Details
MA5-50156
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
20D1
Immunogen
A synthesized peptide derived from Human ATP2A2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3092509
Target Information
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase Ca++ transporting cardiac muscle slow twitch 2; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform; cardiac Ca2+ ATPase; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA; SERCA ATPase; SERCA2; SR Ca(2+)-ATPase 2
Gene Aliases: ATP2A2; ATP2B; DAR; DD; SERCA2
UniProt ID: (Human) P16615
Entrez Gene ID: (Human) 488
calcium-transporting ATPase activity
calcium ion binding
protein binding
ATP binding
protein C-terminus binding
enzyme binding
lutropin-choriogonadotropic hormone receptor binding
S100 protein binding
calcium-transporting ATPase activity involved in regulation of cardiac muscle cell membrane potential
regulation of the force of heart contraction
ER-nucleus signaling pathway
organelle organization
cell adhesion
epidermis development
positive regulation of heart rate
regulation of cardiac muscle contraction by calcium ion signaling
transition between fast and slow fiber
endoplasmic reticulum calcium ion homeostasis
positive regulation of endoplasmic reticulum calcium ion concentration
T-tubule organization
ion transmembrane transport
cellular response to oxidative stress
response to endoplasmic reticulum stress
response to peptide hormone
negative regulation of heart contraction
relaxation of cardiac muscle
sarcoplasmic reticulum calcium ion transport
calcium ion transmembrane transport
regulation of cardiac muscle cell membrane potential
regulation of cardiac muscle cell action potential involved in regulation of contraction
regulation of calcium ion-dependent exocytosis of neurotransmitter
calcium ion transport from cytosol to endoplasmic reticulum
regulation of cardiac conduction
calcium ion import into sarcoplasmic reticulum
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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